Invitrogen™ Malondialdehyde (MDA) Colorimetric Assay Kit, 96 tests

Reliable and sensitive colorimetric assay for lipid peroxidation biomarker quantification

The Invitrogen™ Malondialdehyde (MDA) Colorimetric Assay Kit is a highly reliable, sensitive, and easy-to-use method for quantitative measurement of MDA in serum, plasma, tissue homogenates, and other biological samples. MDA is a key biomarker of oxidative stress and lipid peroxidation, making it an essential target in studies involving oxidative damage, disease mechanisms, and antioxidant evaluation.

This assay is based on the thiobarbituric acid (TBA) reaction: MDA, a catabolite of lipid peroxides, reacts with TBA to form a red adduct with a strong absorption peak at 532 nm, which is measured using a standard microplate reader. The protocol is straightforward, with a total assay time of 65 minutes, requiring minimal hands-on preparation.

Key Features

• High Sensitivity – Detects MDA concentrations as low as 1.13 μmol/L
• Broad Assay Range – 2.92–40 μmol/L for versatile sample measurement
• Simple and Rapid Protocol – Minimal handling with total assay time of 65 minutes
• Versatile Sample Compatibility – Works with serum, plasma, and tissue homogenates
• Reproducible Results – Low intra-assay CV (4.1%) and inter-assay CV (7.2%)
• Convenient Format – 96-test kit suitable for medium- to high-throughput analysis

Applications

• Oxidative stress studies
• Lipid peroxidation quantification in disease models
• Antioxidant efficacy testing
• Metabolic pathway research involving ROS and lipid damage
• Toxicology studies assessing oxidative injury

Assay Principle

1. MDA in samples reacts with thiobarbituric acid (TBA).
2. The reaction forms a stable red compound.
3. The red adduct is measured at 532 nm, and MDA concentration is determined from a standard curve.
4. The Malondialdehyde (MDA) Colorimetric Assay Kit Calculator is used to convert your absorbance readings (usually at 532 nm) into MDA concentrations based on the standard curve you generate during the assay.
   

Specifications

The Invitrogen™ Malondialdehyde (MDA) Colorimetric Assay Kit delivers sensitive, reproducible, and rapid quantification of a critical oxidative stress biomarker. With broad sample compatibility and a simple workflow, it is an ideal choice for oxidative damage research, antioxidant testing, and lipid peroxidation studies.