Thermo Scientific™ Oxoid™ Chromogenic Coliform Agar (ISO)

The Thermo Scientific™ Chromogenic Coliform Agar (Dehydrated) is a highly selective and chromogenic medium designed for the detection, enumeration, and differentiation of coliforms and Escherichia coli in water samples. This medium complies with ISO 9308-1:2014, enabling reliable and efficient microbiological water quality testing in various water sources, including drinking water, pool water, and treatment plant samples.

Key Features

1. ISO 9308-1 Compliance:
   • Conforms to international standards for water quality analysis using membrane filtration.
2. Chromogenic Differentiation:
   • Utilizes Salmon GAL and X-glucuronide to differentiate target organisms:
     • E. coli forms dark blue/violet colonies.
     • Coliforms form pink to red colonies.
3. Gram-Positive Inhibition:
   • Contains Tergitol-7, which inhibits Gram-positive bacteria and non-coliform Gram-negative bacteria.
4. Fast and Efficient:
   • Provides complete detection within 24 hours, eliminating the need for subculturing.
5. Broad Application:
   • Suitable for drinking water, disinfected pool water, and water treatment plant samples.

Applications

1. Water Microbiology

• Enumeration and differentiation of coliforms and E. coli in:
  • Drinking water
  • Recreational water (e.g., swimming pools)
  • Wastewater and treatment plant effluents

2. Environmental Monitoring

• Monitoring microbial water quality in industrial and municipal water systems.

3. Regulatory and Compliance Testing

• Ensures adherence to ISO 9308-1:2014 guidelines for microbiological water testing.

Specifications

Preparation Instructions

1. Suspend: Suspend 26.5g of Chromogenic Coliform Agar in 1 liter of distilled water.
2. Mix and Heat: Heat with frequent agitation until completely dissolved. Do not autoclave.
3. Dispense: Pour the prepared medium into sterile Petri dishes and allow it to solidify.
4. Inoculate: Filter the water sample onto a membrane and transfer the membrane onto the agar surface.
5. Incubate: Incubate at 36 ± 2°C for 24 hours under aerobic conditions.

Advantages

1. Fast Results: Detects and differentiates E. coli and coliforms within 24 hours.
2. Easy Interpretation: Chromogenic differentiation ensures clear identification of target organisms.
3. Selective and Specific: Inhibits non-target organisms while promoting coliform and E. coli recovery.
4. Regulatory Compliance: Ensures reliable results for ISO-standard water testing workflows.
5. Broad Utility: Applicable to multiple water sources, including low bacterial background flora.

Diagnostic Features

Storage and Stability

• Storage: Store the dehydrated medium at 10–30°C in a dry environment.
• Shelf Life: 3 years from the date of manufacture.

Packaging

• Product: Chromogenic Coliform Agar (Dehydrated)
• Quantity: 500g
• Yield: Sufficient for 18.9L of medium

The Thermo Scientific™ Chromogenic Coliform Agar (Dehydrated) is a reliable and efficient solution for the detection, enumeration, and differentiation of coliforms and E. coli in water samples. With its chromogenic properties, rapid results, and compliance with ISO standards, it is an indispensable tool for water quality testing in laboratories focused on public health and environmental monitoring.