Thermo Scientific™ Oxoid™ Baird-Parker Agar Base (Dehydrated)

The Thermo Scientific™ Oxoid™ Baird-Parker Agar Base (Dehydrated) is a highly selective and sensitive medium for the isolation, enumeration, and presumptive identification of Staphylococcus aureus, including coagulase-positive strains, in food and environmental samples. With a carefully balanced formulation, it suppresses non-target bacteria while promoting the recovery and growth of damaged staphylococci, adhering to multiple international food safety standards.

This medium incorporates glycine, lithium, and tellurite for selective inhibition of competing bacteria. Sodium pyruvate and egg yolk emulsion enhance recovery and differentiation, with diagnostic features such as grey-black shiny colonies surrounded by clear zones.

Key Features

1. Selective and Differential:
   • Suppresses growth of competing bacteria with glycine, lithium, and tellurite.
   • Differentiates Staphylococcus aureus through characteristic colony morphology.
2. High Sensitivity:
   • Enables recovery of damaged Staphylococcus aureus cells for accurate results.
3. Diagnostic Clarity:
   • Staphylococcus aureus colonies form grey-black shiny colonies with clear zones due to proteolytic action.
4. Compliance with Standards:
   • Complies with international standards including AFNOR, AOAC, BSI, EP, IDF, ISO, NMKL, and USDA.
5. Versatile Applications:
   • Suitable for food safety, clinical diagnostics, and environmental monitoring.

Applications

1. Food Microbiology

• Detection and enumeration of coagulase-positive Staphylococcus aureus in food products such as meat, dairy, and processed foods.
• Quality control and food safety compliance for manufacturing and processing industries.

2. Clinical and Environmental Microbiology

• Isolation of Staphylococcus aureus from clinical specimens, environmental swabs, and contaminated surfaces.

Specifications

Preparation Instructions

1. Suspend: Suspend 63g of Baird-Parker Agar Base in 1 liter of distilled water.
2. Heat and Dissolve: Heat with frequent agitation until completely dissolved. Do not autoclave.
3. Supplement: Cool to 45–50°C and aseptically add egg yolk emulsion (Oxoid SR0054) and potassium tellurite supplement (Oxoid SR0052).
4. Dispense: Pour the medium into sterile Petri dishes and allow it to solidify.

Diagnostic Features

Advantages

1. High Selectivity: Inhibits non-target bacteria for precise detection of Staphylococcus aureus.
2. Enhanced Recovery: Sodium pyruvate promotes recovery of damaged staphylococci.
3. Ease of Interpretation: Characteristic colony features allow for easy differentiation.
4. Regulatory Compliance: Adheres to international standards, ensuring reliability in food safety testing.
5. Cost-Effective: High yield of 7.9L per 500g package.

Storage and Stability

• Storage: Store the dehydrated medium in a cool, dry environment at 10–30°C.
• Shelf Life: Refer to product label for expiration date.

General References

1. Chopin A., Malcolm S., Jarvis G., et al. (1985). J. Food Protect., 48: 21–27.
2. Baird-Parker A. C. (1962). J. Appl. Bacteriol., 25: 12–19.
3. Zebovitz E., Evans J. B., and Niven C. F. (1955). J. Bacteriol., 70: 686–689.

Packaging

• Product: Baird-Parker Agar Base (Dehydrated)
• Catalog Number: CM1127B
• Quantity: 500g

The Thermo Scientific™ Oxoid™ Baird-Parker Agar Base is a reliable and effective medium for isolating and enumerating coagulase-positive Staphylococcus aureus in food and environmental samples. Its selective and diagnostic properties, high sensitivity, and compliance with international standards make it an essential tool for microbiological testing in food safety, clinical diagnostics, and environmental monitoring.