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Thermo Scientific™ Oxoid™ Egg Yolk Emulsion
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for the identification of Clostridium, Bacillus, and Staphylococcus species by their lipase activity
Specifications:
| Application | Microbiology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Supplement | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Microbiology grade | ||
Thermo Scientific™ Oxoid™ Egg Yolk Emulsion is an additive commonly used in microbiological culture media to aid in the identification and differentiation of bacterial species, particularly in the isolation of Clostridium and Staphylococcus species. Below is a detailed description:
Thermo Scientific™ Oxoid™ Egg Yolk Emulsion
- Product Overview
- Purpose: Used as an enrichment supplement in microbiological culture media.
- Function: Supports the identification of bacterial species by detecting lecithinase and lipase activity, which is key in differentiating Clostridium, Bacillus, and Staphylococcus species.
- Compatibility: Commonly added to media like Baird-Parker Agar for selective isolation.
- Composition
- Ingredients: Prepared from egg yolk, containing natural lecithins and lipids that react with bacterial enzymes.
- Format: Supplied as a sterile 10% emulsion, ready to use.
- Physical Properties
- Appearance: Yellow to pale yellow liquid emulsion.
- Storage Conditions: Store at 2-8°C, away from direct sunlight. Typically has a limited shelf life and should be used within the indicated expiry date.
- Applications
- Microbiological Culture Media Supplement: Enhances media for isolation and differentiation by providing substrates for lecithinase and lipase activity.
- Differentiation of Bacterial Species: Helps identify organisms based on zone formation (opalescence or clearing) around colonies indicating enzyme activity.
- Clinical, Food, and Environmental Testing: Suitable for applications in clinical diagnostics and food and environmental microbiology.
- Usage Guidelines
- Concentration: Typically added at a 10% concentration to culture media as specified in laboratory protocols.
- Sterility: Ready-to-use and sterilized for direct addition to prepared media without further processing.
- Safety and Handling
- Precautions: Non-hazardous but should be handled with standard aseptic techniques to avoid contamination.
- Disposal: Dispose of in accordance with laboratory protocols for biological waste.
Benefits
- Reliable Differentiation: Provides clear visual differentiation of lecithinase-positive and lipase-positive bacteria.
- Convenience: Pre-sterilized and ready to use, simplifying media preparation.
This emulsion is an essential component for selective and differential microbiology applications, ensuring accurate identification of certain bacterial species based on enzyme activity.