Applied Biosystems™ TaqMan™ SNP Genotyping Assay, human

The Applied Biosystems™ TaqMan™ SNP Genotyping Assay (Human) is a gold-standard solution for the accurate detection and discrimination of single nucleotide polymorphisms (SNPs) in genomic DNA. Designed using advanced TaqMan™ 5′-nuclease chemistry, each assay enables precise genotyping of a specific SNP through real-time PCR.

These assays are part of a comprehensive, genome-wide collection covering over 4.5 million human SNP targets, including variants from the 1000 Genomes Project, HapMap, and coding regions, making them highly relevant for a wide range of research applications.

Key Features

• Gold-Standard TaqMan™ Chemistry
  Proven performance for accurate, reproducible SNP genotyping.
• Dual-Labeled Probe System
  • VIC™ dye detects Allele 1
  • FAM™ dye detects Allele 2
    Enables clear allelic discrimination.
• Single-Tube Format
  Simplifies workflow and minimizes pipetting errors.
• Pre-Designed & Validated Assays
  Functionally tested to ensure reliable results.
• Extensive SNP Coverage
  Access to millions of genome-wide human SNP assays.
• No Optimization Required
  Ready-to-use format for rapid deployment in experiments.

Applications

• SNP genotyping and allelic discrimination
• Disease association studies
• Pharmacogenomics and drug metabolism research
• Population genetics and stratification studies
• Linkage mapping and biomarker discovery
• Genomic and translational research

Principle of the Assay

The assay utilizes TaqMan™ 5′-nuclease PCR chemistry:

• Two sequence-specific primers amplify the target DNA
• Two allele-specific probes labeled with VIC™ and FAM™ dyes bind selectively
• During PCR, probe cleavage releases fluorescence
• Fluorescent signals enable accurate genotype determination

Technical Specifications

Packaging & Ordering Information

Workflow Requirements

Each genotyping reaction requires:

• Purified genomic DNA (1–20 ng)
• TaqMan™ SNP Genotyping Assay
• TaqMan™ Genotyping Master Mix (sold separately, e.g., Cat. No. 4371355)

Instrument Compatibility

Compatible with Applied Biosystems™ platforms, including:

• 7500 / 7500 Fast Systems
• StepOne™ / StepOnePlus™
• ViiA™ 7 System
• QuantStudio™ Series (3, 5, 6 Flex, 7, 12K Flex, Pro)
• QuantStudio™ Absolute Q Digital PCR System
• ProFlex™, VeritiPro™, SimpliAmp™, MiniAmp™ Thermal Cyclers*

*Pre-read and post-read fluorescence detection required on real-time PCR systems when using standard thermal cyclers.

Performance Highlights

• High allelic discrimination accuracy
• Consistent and reproducible results
• Optimized assay design from extensive bioinformatics pipeline
• Backed by >40,000 scientific publications and 350+ patents

The Applied Biosystems™ TaqMan™ SNP Genotyping Assay (Human) provides a robust and reliable solution for SNP analysis, offering high accuracy, ease of use, and extensive genomic coverage. Its validated performance and streamlined workflow make it an essential tool for modern genomics, molecular biology, and population genetics research.