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Applied Biosystems™ TaqPath™ ProAmp™ Master Mix
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Multiplex SNP Genotyping and Purified DNA-Based CNV Analysis
Specifications:
| Application | Gene Expression, Multiplex SNP analysis, Pharmacogenomics research, Copy number variation analysis | ||
| Storage Temperature | 2-8°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
The Applied Biosystems™ TaqPath™ ProAmp™ Master Mix is a high-performance qPCR master mix designed for multiplex SNP genotyping and copy number variation (CNV) analysis. It is optimized for use with TaqMan™ SNP Genotyping Assays and supports accurate, reproducible amplification from purified genomic DNA as well as crude lysate inputs for SNP genotyping workflows.
The formulation is engineered to provide strong inhibitor tolerance, making it suitable for sample types commonly associated with PCR inhibition, including blood, buccal swabs, and card punches. For CNV analysis, the use of purified genomic DNA is recommended to ensure accurate copy number determination.
Manufactured under an ISO 13485 quality management system, TaqPath™ ProAmp™ Master Mix is suitable for high-throughput research environments requiring consistent lot-to-lot performance, reliable data quality, and workflow compatibility with Applied Biosystems™ real-time PCR platforms.
Key Features
- qPCR master mix for multiplex SNP genotyping
- Supports SNP genotyping from purified genomic DNA or crude lysates
- Suitable for copy number variation analysis using purified genomic DNA
- Compatible with TaqMan™ SNP Genotyping Assays
- Supports singleplex and multiplex SNP detection workflows
- Very high inhibitor tolerance for challenging biological samples
- Designed for blood, buccal swab, and card punch-derived samples
- Contains dUTP and UNG/UDG for contamination control workflows
- Premixed with ROX passive reference dye
- Compatible with fast or standard qPCR cycling methods
- 72-hour assembled reaction stability with purified DNA
- Manufactured in an ISO 13485-certified facility
- Compliance documentation package available
Applications
- SNP genotyping
- Multiplex SNP analysis
- Allelic discrimination studies
- Copy number variation analysis
- Pharmacogenomics research
- Genetic variation studies
- High-throughput qPCR workflows
- Crude lysate-based genotyping
- Purified genomic DNA-based qPCR
- Research and regulated laboratory environments
Workflow Compatibility
TaqPath™ ProAmp™ Master Mix forms part of a streamlined Sample-to-Genotype workflow and can be used with:
| Workflow Component | Example |
|---|---|
| Sample Preparation | DNA Extract All™ Reagents Kit |
| Assays | TaqMan™ SNP Genotyping Assays |
| Genotyping Assays | TaqMan™ DME Genotyping Assays |
| Real-Time PCR Platforms | QuantStudio™ Systems, StepOne™, ViiA™ 7 |
| Analysis Software | Diomni™ Design and Analysis Software |
Product Benefits
| Benefit | Description |
|---|---|
| High Data Quality | Supports specific, reproducible SNP genotyping and CNV analysis |
| Inhibitor Tolerance | Performs reliably with challenging sample-derived inputs |
| Crude Lysate Compatibility | Enables SNP genotyping without DNA purification |
| Multiplex Flexibility | Supports singleplex and multiplex SNP workflows |
| Workflow Efficiency | Suitable for automated and high-throughput qPCR workflows |
| Manufacturing Consistency | Produced under ISO 13485 quality controls |
| Compliance Support | Quality and regulatory documentation available |
Instrument Compatibility
The master mix is suitable for use with the following Applied Biosystems™ real-time PCR systems:
| Compatible Instruments |
|---|
| 7500 Fast Real-Time PCR System |
| QuantStudio™ 3 Real-Time PCR System |
| QuantStudio™ 5 Real-Time PCR System |
| QuantStudio™ 6 Flex Real-Time PCR System |
| QuantStudio™ 7 Flex Real-Time PCR System |
| QuantStudio™ 12K Flex Real-Time PCR System |
| ViiA™ 7 Real-Time PCR System |
| ViiA™ 7 Dx System |
| QuantStudio™ Dx System |
| StepOne™ Real-Time PCR System |
| StepOnePlus™ Real-Time PCR System |
Technical Specifications
| Parameter | Specification |
|---|---|
| Brand | Applied Biosystems™ |
| Product Line | TaqPath™ |
| Product Name | TaqPath™ ProAmp™ Master Mix |
| Product Type | qPCR Master Mix |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Main Application | Genotyping |
| Multiplex Capability | 4-plex |
| Passive Reference Dye | ROX, premixed |
| Polymerase | Taq DNA Polymerase |
| Includes | dUTP, UNG/UDG |
| Inhibitor Tolerance | Very high |
| Target Specificity | cDNA or gDNA only |
| Reaction Speed | Fast or standard |
| Thermostability | 72 hours, assembled reaction |
| Shipping Condition | Wet ice |
| Storage | 2°C to 8°C, protected from light |
| Unit Size | Each |
Ordering Information
| Catalog Number | Quantity |
|---|---|
| A30865 | 1 × 1 mL |
| A30866 | 1 × 10 mL |
| A30871 | 2 × 10 mL |
| A30867 | 1 × 50 mL |
| A30872 | 2 × 50 mL |
Storage & Handling
Store the product at 2°C to 8°C and protect it from light. Shipments are typically supplied on wet ice. For best performance, follow the recommended storage and handling conditions and avoid unnecessary exposure to light or temperature fluctuations.
The Applied Biosystems™ TaqPath™ ProAmp™ Master Mix is a robust and reliable qPCR reagent for laboratories performing high-throughput SNP genotyping and purified DNA-based CNV analysis. Its strong inhibitor tolerance, multiplex capability, compatibility with Applied Biosystems™ real-time PCR platforms, and ISO 13485 manufacturing quality make it a dependable choice for genetic analysis workflows requiring sensitivity, reproducibility, and operational efficiency.
- Pack Size: 1 x 1 mL, sufficient for 200 Rxns 1 x 10 mL, sufficient for 2,000 Rxns 2 x 10 mL, sufficient for 4,000 Rxns 1 x 50 mL, sufficient for 10,000 Rxns 2 x 50 mL, sufficient for 20,000 Rxns