Abbexa Human Haptoglobin Related Protein (HPR) ELISA Kit, 96 Tests

High-Sensitivity, Quantitative ELISA for HPR Detection in Serum, Plasma, and Biological Fluids

The Abbexa Human Haptoglobin Related Protein (HPR) ELISA Kit is a high-sensitivity, quantitative sandwich ELISA assay designed for accurate measurement of HPR concentrations in serum, plasma, and other biological fluids. This assay ensures high specificity, minimal cross-reactivity, and excellent reproducibility, making it an essential tool for biomedical research, disease biomarker studies, and diagnostic applications.

With a detection range of 78 pg/mL – 5000 pg/mL and a sensitivity of <35 pg/mL, this ELISA kit is optimized for high-precision quantification of HPR in human samples.

Key Features & Benefits

✅ High Sensitivity (Detection Limit: <35 pg/mL) – Ensures precise and reliable HPR quantification

✅ Wide Detection Range (78 pg/mL – 5000 pg/mL) – Suitable for various sample concentrations

✅ Sandwich ELISA Technology for Specific HPR Detection – Reduces non-specific binding & false positives

✅ Minimal Cross-Reactivity & Interference – Ensures high specificity for Haptoglobin Related Protein (HPR)

✅ Pre-Coated 96-Well Microplate for Efficient Testing – Simplifies assay workflow & data collection

✅ Highly Stable & Reproducible – Intra-Assay CV <10% & Inter-Assay CV <12%

✅ Colorimetric Detection (450 nm) – Compatible with standard microplate readers

✅ Validated for Serum, Plasma, & Biological Fluids – Suitable for clinical & research applications

Technical Specifications

Assay Principle

The Abbexa HPR ELISA Kit is based on sandwich enzyme-linked immunosorbent assay (ELISA) technology:

1️⃣ Capture Antibody Pre-Coating – The microplate is pre-coated with an HPR-specific antibody.

2️⃣ Sample & Standard Addition – Standards, diluted samples, and control solutions are added to designated wells and incubated.

3️⃣ Binding & Detection – Biotin-conjugated secondary antibody is added, followed by HRP-conjugated reagent to amplify the signal.

4️⃣ Enzymatic Reaction – TMB substrate is added, producing a blue color proportional to the amount of HPR present.

5️⃣ Colorimetric Readout at 450 nm – Stop solution is added, turning the solution yellow. The intensity is measured using a microplate reader.

Kit Components

✔ Pre-Coated 96-Well Microplate

✔ Lyophilized Standard

✔ Standard Diluent Buffer

✔ Wash Buffer

✔ Detection Reagent A (Biotin-Conjugated Antibody)

✔ Detection Reagent B (HRP-Conjugated Antibody)

✔ Diluent A & Diluent B

✔ TMB Substrate Solution

✔ Stop Solution

✔ Plate Sealer

Materials Required But Not Provided

🔹 37°C incubator

🔹 Multichannel & single-channel pipettes with sterile tips

🔹 Microplate washer or squirt bottle

🔹 Distilled water & absorbent filter paper

🔹 Microplate reader (450 nm wavelength)

🔹 ELISA shaker for uniform incubation

Assay Procedure

1️⃣ Equilibrate all reagents and samples to room temperature (18-25°C) before starting the assay.

2️⃣ Set up standard, test samples, and control wells on the pre-coated plate.

3️⃣ Add 100 µL of each diluted sample/standard into their respective wells. Incubate for 1 hour at 37°C.

4️⃣ Add 100 µL of Detection Reagent A to each well and incubate for 1 hour at 37°C.

5️⃣ Wash plate 3 times, then add 100 µL of Detection Reagent B. Incubate for 30 minutes at 37°C.

6️⃣ Wash plate 5 times, then add 90 µL of TMB Substrate and incubate for 10-20 minutes at 37°C.

7️⃣ Stop the reaction with 50 µL of Stop Solution and measure optical density (OD) at 450 nm.

Results Calculation & Interpretation

• Average the O.D.450 duplicate readings for each sample and standard.
• Subtract the blank (zero) O.D.450 reading from each value.
• Construct a standard curve by plotting O.D.450 vs. concentration (pg/mL).
• Interpolate sample HPR concentrations from the standard curve.

Performance Validation

Precision & Accuracy

• Standards & samples were tested in duplicate across multiple plates, ensuring reproducibility & minimal variation.

Applications

🧬 Biomarker Discovery & Disease Research – Detects HPR levels in disease models & clinical studies

🔬 Hematology & Inflammatory Response Studies – Evaluates HPR expression in blood samples

🦠 Infectious Disease & Immunology Research – Investigates HPR-related immune responses

⚕ Clinical & Translational Medicine – Supports HPR-based diagnostic and therapeutic research

📊 Pharmaceutical & Drug Development – Used in biological fluid analysis for drug efficacy testing

Ordering Information

Regulatory & Safety Information

⚠️ For Research Use Only (RUO). Not intended for clinical or diagnostic applications.

⚠️ Follow Laboratory Safety Procedures – Use PPE (gloves, lab coat, eye protection) when handling reagents.

⚠️ Store Kit at 4°C Upon Arrival – Avoid repeated freeze-thaw cycles.

Why Choose Abbexa Human HPR ELISA Kit?

🔹 High Sensitivity (Detection Limit <35 pg/mL) for Accurate Quantification

🔹 Validated for Serum, Plasma, and Biological Fluids

🔹 Pre-Coated 96-Well Microplate for Easy Setup

🔹 Minimal Cross-Reactivity for Specific HPR Detection

🔹 Reliable & Reproducible Assay (Intra-Assay CV <10%)

For bulk orders, technical support, or additional information, contact LabMart Limited today. 🚀