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Gibco™ ExpiFectamine™ Sf Transfection Reagent
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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Next-generation transfection reagent optimized for Sf9 and Sf21 insect cells
The Gibco™ ExpiFectamine™ Sf Transfection Reagent is a high-performance, cationic lipid-based reagent engineered for efficient transfection of insect cells, particularly Sf9 and Sf21 cell lines. Optimized as a core component of the ExpiSf™ Expression System, it enables rapid, reproducible delivery of plasmid DNA into insect cells for the production of high-titer recombinant baculovirus (P0) without the need for virus amplification.
This reagent supports both adherent and suspension cultures, with a simplified workflow that includes a short 5-minute complexation time, low cytotoxicity, and no post-transfection medium change, ensuring maximum efficiency and user convenience.
Key Features
- Robust & Reproducible: Delivers consistent transfection performance for confident experimental results.
- P0 Baculovirus Production: Enables efficient generation of high-quality, high-titer P0 recombinant baculovirus.
- Flexible Culture Compatibility: Effective in adherent and suspension cultures of Sf9 and Sf21 cells.
- Low Toxicity: Gentle on cells; medium replacement after transfection is not required.
- Fast Workflow: Features a 5-minute DNA complexation step for rapid protocol execution.
- Scalable: Linearly scalable transfection from 4 mL to 500 mL culture volumes in suspension format.
- Compatible Media: Validated with ExpiSf CD Medium, Sf-900™ II SFM, and Sf-900™ III SFM.
Applications
- Transient transfection of insect cells (Sf9, Sf21)
- Recombinant baculovirus (P0) generation
- Gene expression studies in insect cell systems
- Protein production using the baculovirus expression vector system (BEVS)
Technical Specifications
| Specification | Details |
|---|---|
| Product Type | Transfection Reagent |
| Transfection Technique | Lipid-based |
| Cell Type | Insect cells (Sf9, Sf21) |
| Sample Type | Plasmid DNA, Baculovirus DNA |
| Serum Compatible | Yes |
| High-throughput Compatible | Yes |
| Product Line | ExpiFectamine™ |
| Shipping Conditions | Room temperature or wet ice |
| Storage Conditions | Store at 2–8°C, do not freeze |
| Shelf Life | 24 months from date of manufacture |
| Unit Size | 1 mL |
| Catalog Number | A38915 |
The Gibco™ ExpiFectamine™ Sf Transfection Reagent is an essential tool for researchers using baculovirus-based protein expression in insect cell systems. Its ease of use, high efficiency, and compatibility with both suspension and adherent cultures make it a superior choice for labs requiring reproducible, scalable transfection solutions.