Gibco™ ExpiSf9™ Cells, 1.0 x 107 cells/mL

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Conditions générales
Garantie satisfait ou remboursé de 30 jours
Expédition : 2-3 jours ouvrables

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    Specifications:
    Application Cloning, Transfection
    Storage Temperature Store Cells in Liquid Nitrogen untill ready to use
    Product Type cells Forms Liquid
    Product Brand Thermo Fisher Scientific™, Gibco
    Product Grade Cell Culture

    High-Density Suspension Insect Cells for Baculovirus Expression Systems

    Gibco™ ExpiSf9™ Cells are a non-engineered derivative of the classical Sf9 insect cell line (Spodoptera frugiperda), adapted for robust high-density suspension growth in Gibco™ ExpiSf™ CD Medium. As a central component of the ExpiSf™ Expression System, these cells are optimized for high-yield recombinant protein production using the baculovirus expression vector system (BEVS).

    Engineered for scalability, consistency, and high performance, ExpiSf9™ Cells enable researchers and biomanufacturing teams to achieve significantly higher protein yields per liter compared to traditional insect cell lines.

    Key Features and Benefits

    • High-Yield Protein Expression
      Achieve up to 3× more recombinant protein per liter than conventional Sf9 cells when used with the ExpiSf™ Expression System.
    • High-Density Suspension Growth
      Reach cell densities up to 2 × 10⁷ cells/mL, enabling increased productivity in limited volumes.
    • Rapid Growth and Recovery
      Post-thaw recovery is fast with doubling times of ~24 hours, reducing lag time between revival and production.
    • Extended Culture Viability
      Maintain consistent expression levels for over 20 passages post-thaw.
    • Scalable Culture Format
      Perform expression workflows in culture volumes from 4 mL (deep-well plates) up to >3 L (shake flasks or bioreactors).

    Product Composition and Format

    • 1 vial (1.5 mL) of cryopreserved ExpiSf9™ Cells
    • Viable cell density: 1 × 10⁷ cells/mL
    • Cryopreserved in ExpiSf™ CD Medium with 7.5% DMSO
    • Supplied on dry ice, store in vapor-phase liquid nitrogen
    • Intended for use with ExpiSf™ CD Medium and the ExpiSf™ Expression System only

    Recommended Applications

    • Recombinant protein expression using BEVS
    • Structural biology, vaccine research, and antibody fragment production
    • Academic, biotech, and CRO/CDMO protein production workflows
    • Use in research-scale and preclinical development environments

    Storage and Handling

    ConditionRequirement
    ShippingDry ice
    StorageVapor-phase liquid nitrogen
    Thawing MediumGibco™ ExpiSf™ CD Medium
    Incubation ConditionsNon-humidified, non-CO₂, 27–28°C incubator
    Recommended FormatSuspension culture only

    Note: ExpiSf9™ Cells are not recommended for adherent culture formats or routine passaging on plates.

    Ordering Information

    Catalog NumberDescriptionQuantityList Price (GBP)
    A35243ExpiSf9™ Insect Cells (cryopreserved)1 vial£806.00


    Gibco™ ExpiSf9™ Cells offer a robust, scalable platform for insect-based protein expression, ideal for research and early development workflows requiring high yield, reproducibility, and compatibility with modern BEVS tools. When paired with the ExpiSf™ Expression System, these cells unlock powerful productivity gains in recombinant protein production.

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