Gibco™ ExpiSf9™ Cells, 1.0 x 107 cells/mL

High-Density Suspension Insect Cells for Baculovirus Expression Systems

Gibco™ ExpiSf9™ Cells are a non-engineered derivative of the classical Sf9 insect cell line (Spodoptera frugiperda), adapted for robust high-density suspension growth in Gibco™ ExpiSf™ CD Medium. As a central component of the ExpiSf™ Expression System, these cells are optimized for high-yield recombinant protein production using the baculovirus expression vector system (BEVS).

Engineered for scalability, consistency, and high performance, ExpiSf9™ Cells enable researchers and biomanufacturing teams to achieve significantly higher protein yields per liter compared to traditional insect cell lines.

Key Features and Benefits

• High-Yield Protein Expression
  Achieve up to 3× more recombinant protein per liter than conventional Sf9 cells when used with the ExpiSf™ Expression System.
• High-Density Suspension Growth
  Reach cell densities up to 2 × 10⁷ cells/mL, enabling increased productivity in limited volumes.
• Rapid Growth and Recovery
  Post-thaw recovery is fast with doubling times of ~24 hours, reducing lag time between revival and production.
• Extended Culture Viability
  Maintain consistent expression levels for over 20 passages post-thaw.
• Scalable Culture Format
  Perform expression workflows in culture volumes from 4 mL (deep-well plates) up to >3 L (shake flasks or bioreactors).

Product Composition and Format

• 1 vial (1.5 mL) of cryopreserved ExpiSf9™ Cells
• Viable cell density: 1 × 10⁷ cells/mL
• Cryopreserved in ExpiSf™ CD Medium with 7.5% DMSO
• Supplied on dry ice, store in vapor-phase liquid nitrogen
• Intended for use with ExpiSf™ CD Medium and the ExpiSf™ Expression System only

Recommended Applications

• Recombinant protein expression using BEVS
• Structural biology, vaccine research, and antibody fragment production
• Academic, biotech, and CRO/CDMO protein production workflows
• Use in research-scale and preclinical development environments

Storage and Handling

Note: ExpiSf9™ Cells are not recommended for adherent culture formats or routine passaging on plates.

Ordering Information

Gibco™ ExpiSf9™ Cells offer a robust, scalable platform for insect-based protein expression, ideal for research and early development workflows requiring high yield, reproducibility, and compatibility with modern BEVS tools. When paired with the ExpiSf™ Expression System, these cells unlock powerful productivity gains in recombinant protein production.