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Thermo Scientific™ Pierce™ Protein Refolding Kit
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Specifications:
| Application | Protein Assays & Analysis | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Pierce™ Protein Refolding Kit enables systematic screening and optimization of protein refolding conditions for recombinant proteins expressed as inclusion bodies. The kit utilizes a matrix-based design to evaluate key buffer components—denaturants, redox agents, and additives—across multiple concentration levels, helping to identify conditions that suppress aggregation and restore native protein conformation and function.
This kit includes high-purity reagents, flexible matrix components, and a detailed refolding guide for tailoring experiments to a wide variety of target proteins. It is ideal for labs working with proteins expressed in E. coli or other systems where inclusion body formation is common.
Key Features and Benefits
- Matrix Strategy Design – Nine buffer combinations allow screening across redox and denaturant conditions
- Customizable Conditions – Adjustable parameters for specific protein folding requirements
- High-Purity Reagents – Ensures reproducibility and minimizes contamination
- Efficient Refolding – Reduces trial-and-error and optimizes protein yield and activity
- Comprehensive Protocol – Includes guidance for inclusion body isolation, solubilization, and activity measurement
Applications
- Refolding of proteins from inclusion bodies
- Optimization of protein folding for biochemical/structural studies
- Evaluation of redox environments for disulfide bond reformation
- Refolding assay development for enzymes and antibody fragments
- Pilot screening for large-scale protein production
Specifications
| Attribute | Details |
|---|---|
| Format | Liquid and powder reagents |
| No. of Reactions | 100 reactions |
| Reagent Type | Denaturants, redox agents, folding additives |
| Target | Denatured recombinant proteins |
| Product Line | Pierce™ |
| Storage | –20°C (or 4°C for up to 2 months) |
| Shipping Condition | Dry ice |
Kit Contents
- Base refolding buffers
- Additives (e.g., L-arginine, GSH/GSSG)
- High-purity chaotropes and denaturants
- Refolding Guide with matrix setup and analysis recommendations
Related Products
- Pierce™ Inclusion Body Solubilization Reagent
- Thermo Scientific™ BCA Protein Assay Kit
- Pierce™ Desalting Spin Columns
- Thermo™ Enzyme Activity Assay Kits
The Pierce™ Protein Refolding Kit is a powerful and flexible solution for restoring function to recombinant proteins expressed as inclusion bodies. With a customizable matrix format and complete buffer system, it allows researchers to recover high yields of functional protein in a reproducible and efficient manner—ideal for protein structure-function studies, assay development, and drug screening applications.