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Specifications:
| Application | molecular biology | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Dithiothreitol (DTT) is a reducing agent widely used in biological research for its ability to break disulfide bonds in proteins and peptides. This property makes it particularly useful in applications such as kinase assays, protein preparation, and high-throughput screening. Invitrogen's DTT is provided as a 1M liquid solution in water, tested to ensure minimal background fluorescence, optimizing its use in fluorescence-based assays like LanthaScreen® Technology.
Key Features
- High Purity for Assay Sensitivity
- Specifically tested for low background fluorescence, making it suitable for high-throughput screening (HTS) and fluorescence-based assays.
- Ready-to-Use
- Provided as a 1M liquid solution in water, eliminating the need for preparation and minimizing errors during dilution.
- Optimal for Kinase Assays
- Essential for LanthaScreen® Technology, where maintaining a reducing environment is critical for assay performance.
- Convenient Packaging
- Packaged in a 1 mL volume, ideal for precise experimental setups in small-scale and HTS workflows.
- Research-Grade
- For research use only, ensuring its suitability for molecular biology and biochemistry applications but not for diagnostic or clinical purposes.
Applications
- Kinase Assays
- Ensures proper assay function by maintaining reducing conditions to preserve enzyme activity and prevent disulfide bond formation.
- Protein Preparation
- Reduces disulfide bonds in proteins and peptides, aiding in denaturation and refolding experiments.
- High-Throughput Screening (HTS)
- Provides consistent performance in fluorescence-based HTS for drug discovery and biochemical studies.
- LanthaScreen® Technology
- Integral component for reducing conditions in TR-FRET assays that measure kinase and phosphatase activity.
- General Reducing Agent
- Used in SDS-PAGE, proteomics, and enzymatic reactions where disulfide reduction is necessary.
Specifications
| Parameter | Details |
|---|---|
| Product Type | Dithiothreitol (DTT) |
| Concentration | 1M |
| Form | Liquid |
| Unit Size | 1 mL |
| Shipping Condition | Dry Ice |
| Storage Condition | -20°C |
| Application | Kinase assays, HTS, reducing agent |
Handling and Storage
- Shipping: Delivered on dry ice to preserve stability during transport.
- Storage: Keep at -20°C to maintain product integrity.
- Usage: Thaw before use and return to the freezer immediately after dispensing to avoid degradation.
Precautions
- For research use only; not intended for diagnostic or therapeutic applications.
- Handle with care to avoid contamination and maintain assay sensitivity.
Advantages
- Fluorescence Tested: Minimizes interference in fluorescence-based assays.
- Convenience: Ready-to-use liquid form eliminates preparation errors.
- Versatility: Suitable for various biochemical and molecular biology applications.
- Reliability: Tested for consistency, ensuring reproducible results in sensitive assays.
Invitrogen™ DTT, 1M (1 mL) is a highly reliable reducing agent tailored for advanced biochemical applications, including kinase assays and high-throughput screening. Its fluorescence-tested formulation and ready-to-use format make it indispensable for researchers requiring precision and reproducibility in sensitive experimental setups.