Thermo Scientific™ GlcNAz (N-azidoacetylglucosamine tetraacylated)

Azide-Labeled Sugar for Metabolic Glycoprotein Labeling & Click Chemistry

Thermo Scientific™ Pierce GlcNAz (N-Azidoacetylglucosamine-Tetraacylated) is a bioorthogonal, azide-labeled sugar designed for highly specific glycoprotein research via in vivo metabolic labeling and chemoselective ligation. This reagent is a metabolic analog of N-acetylglucosamine (GlcNAc) that allows direct tagging of glycoproteins in post-translational glycosylation pathways for detection, conjugation, and purification.

GlcNAz enables cellular incorporation of azide groups into O-linked glycosylation (O-GlcNAc-modified proteins), making it a powerful tool for glycobiology research, proteomics, and cellular pathway investigations.

Key Features & Benefits

✅ Bioorthogonal Labeling – The azide group is small, nonreactive, and absent from biological systems, preventing interference with endogenous cellular functions.

✅ Metabolic Incorporation – Mimics natural GlcNAc sugars, allowing enzymatic incorporation into glycoproteins via normal cellular glycosylation pathways.

✅ Click Chemistry Compatibility – Reacts selectively with alkyne-functionalized compounds via copper-free click chemistry or phosphine-based Staudinger ligation.

✅ Highly Specific Detection – Enables precise tracking and purification of glycosylated proteins using fluorescent dyes, biotin reagents, or affinity tags.

✅ Compatible with O-Linked Glycosylation Pathways – Specifically labels O-GlcNAc and O-GalNAc-modified proteins, distinguishing it from N-linked glycosylation analogs.

✅ Versatile Research Applications – Supports glycoproteomics, proteomics, cellular imaging, and immunoprecipitation-based assays.

✅ Stable & Easy to Use – Soluble in DMSO, stored at -20°C, and shipped at ambient temperature for convenience.

Applications

🔬 Metabolic Glycoprotein Labeling – Enables selective tagging of glycoproteins in live cells

🧪 Click Chemistry-Based Detection & Imaging – Reacts with alkyne-functionalized fluorophores for visualization

🦠 O-Linked Glycosylation Studies – Labels O-GlcNAc and O-GalNAc glycoproteins

⚗️ Proteomics & Glycoproteomics – Supports protein identification and pathway analysis

🛠 Biotinylation & Protein Purification – Enables affinity-based enrichment of glycoproteins

Technical Specifications

Mechanism of Action & Glycosylation Pathways

Glycoprotein Labeling & Detection Process:

1️⃣ GlcNAz is incorporated into cellular glycoproteins via endogenous glycosylation pathways.

2️⃣ Azide-functionalized glycoproteins are labeled with alkyne or phosphine reagents through bioorthogonal click chemistry (Cu-free).

3️⃣ Conjugated molecules (e.g., fluorescent dyes, biotin tags) allow visualization, detection, and purification.

N-Linked vs. O-Linked Glycosylation Pathways:

• O-Linked Glycosylation (O-GlcNAc & O-GalNAc) – Labeled by GlcNAz & GalNAz
• N-Linked Glycosylation – Labeled by ManNAz (N-Azidoacetylmannosamine)

Related Products

Recommended Experimental Workflow

1. Prepare Cell Culture – Maintain cells in standard conditions with complete media.
2. Metabolic Incorporation – Supplement media with GlcNAz (N-azidoacetylglucosamine) to allow cellular uptake and glycoprotein labeling.
3. Cell Processing – After the desired labeling period, cells are lysed or fixed for analysis.
4. Click Chemistry or Staudinger Ligation – React azide-labeled glycoproteins with alkyne-functionalized fluorophores, biotin, or affinity tags.
5. Detection & Analysis – Use fluorescence microscopy, Western blotting, mass spectrometry, or flow cytometry to detect labeled glycoproteins.

Regulatory & Safety Information

⚠️ For Research Use Only. Not for diagnostic or therapeutic applications.

⚠️ Handle with Caution – Use protective gloves, eyewear, and lab coats when handling the reagent.

⚠️ Dispose of in accordance with chemical safety regulations.

Why Choose Thermo Scientific™ Pierce GlcNAz?

🔹 Highly Specific Glycoprotein Labeling – Enables selective tagging of glycosylated proteins

🔹 Compatible with Click Chemistry & Staudinger Ligation – Ensures versatile conjugation options

🔹 Stable & Easy to Use – DMSO-soluble and stored at -20°C for long-term stability

🔹 Supports Advanced Glycobiology & Proteomics Research – Ideal for cellular imaging, Western blotting, and mass spectrometry

For bulk orders, technical support, or additional information, contact LabMart Limited today.