Liofilchem G.C Medium

The Liofilchem™ G.C. Medium is a basal culture medium specifically formulated for the isolation and cultivation of Haemophilus spp. and Neisseria spp.. When supplemented with appropriate selective and enrichment additives, such as Haemophilus Supplement, V.C.N. Supplement, or Vitalex Growth Supplement, it provides a reliable environment for the growth of gonococci, meningococci, and Haemophilus species.

Key Features

1. Selective and Enrichment Capabilities:
   • Can be tailored for specific microorganisms using supplements like V.C.N., V.C.N.T., and Haemophilus supplements.
2. Optimized for Fastidious Organisms:
   • Supports the growth of Neisseria gonorrhoeae, Neisseria meningitidis, and Haemophilus influenzae.
3. Validated for Clinical Applications:
   • Tested with control strains to ensure reliability in clinical microbiology.
4. USP and CLSI Compliance:
   • Conforms to international standards for microbiological testing.

Typical Formula (g/L)

Applications

1. Clinical Microbiology:
   • Detection and cultivation of Haemophilus influenzae, Neisseria gonorrhoeae, and Neisseria meningitidis from clinical specimens.
2. Research:
   • Studies on fastidious organisms, including Neisseria and Haemophilus spp.
3. Environmental and Food Microbiology:
   • Monitoring contamination by fastidious organisms in water, food, and environmental samples.

Preparation Instructions

1. Prepare the Base Medium:
   • Suspend 43.0 g of G.C. Medium powder in 1 liter of distilled or deionized water.
   • Heat to dissolve completely and autoclave at 121°C for 15 minutes.
2. Enrich and Supplement:
   • Cool the medium to 45-50°C, then aseptically add:
     • 5-10% defibrinated horse blood (Code 83396).
     • 2 vials of Vitalex Growth Supplement (Code 81023), reconstituted with Vitalex Restoring Solution.
     • Appropriate selective supplements (e.g., Haemophilus Supplement, V.C.N., or V.C.N.T. supplements).
3. Pour Plates:
   • Mix thoroughly and pour into sterile Petri dishes.

Supplement Options

1. Haemophilus Supplement (Code 81014):
   • Selective for Haemophilus spp., containing Bacitracin, Vancomycin, and Clindamycin.
2. V.C.N. Supplement (Code 81022):
   • Selective for Neisseria spp., containing Vancomycin, Colistin, and Nystatin.
3. V.C.N.T. Supplement (Code 81024):
   • For rectal-origin specimens, includes Trimethoprim to inhibit Proteus spp.
4. Vitalex Growth Supplement (Code 81023):
   • Provides vitamins, amino acids, and cofactors for fastidious organisms.

Technique

1. Inoculate plates using a swab or streaking method to ensure isolated colonies.
2. Incubate at 36 ± 1°C in a sealed jar with 5-10% CO₂ and 70% humidity for 24-48 hours.
3. Examine plates for characteristic colony growth.

Quality Control

Warnings and Precautions

1. For in vitro diagnostic use only.
2. Follow standard aseptic techniques during handling and preparation.
3. Dispose of waste according to local and national regulations.

Storage and Shelf Life

• Dehydrated Medium:
  • Store at 10–30°C in its original container.
• Prepared Plates:
  • Store at 2–8°C, protected from light.
• Shelf Life:
  • Valid until the expiry date indicated on the label.

Advantages

• Highly Selective and Nutrient-Rich:
  • Supports fastidious organisms while inhibiting non-target microbes.
• Customizable:
  • Tailor for specific organisms using selective and enrichment supplements.
• Cost-Effective:
  • Versatile medium for clinical and research applications.

Packaging

References

1. Chapin, C.K., G.V. Doern (1983). J. Clin. Microbiol. 17:1163–1165.
2. Martin, J.E., Armstrong J.H., Smith P.B. (1974). Appl. Microbiol. 27:802–805.
3. NCCLS M22-A2 (1996). Quality Assurance for Commercially Prepared Microbiological Culture Media.
4. Seth, A. (1970). Brit. J. Vener. Dis. 46:201–202.

Why Choose Liofilchem G.C. Medium?

• Proven Reliability: Trusted for the isolation of fastidious organisms like Haemophilus and Neisseria spp.
• Flexible Applications: Suitable for clinical diagnostics, research, and environmental monitoring.
• Validated Performance: Ensures reliable and reproducible results with standard control strains.