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Invitrogen™ Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP, 2mL
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Specifications:
| Application | Western Blot, ELISA-Based Assays, Immunohistochemistry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | antibody | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP is a high-quality polyclonal secondary antibody, specifically designed to detect and bind to rabbit IgG (H+L) primary antibodies in Western blot (WB), immunohistochemistry (IHC), and ELISA applications. This antibody is conjugated to Horseradish Peroxidase (HRP), making it ideal for chemiluminescent, colorimetric, and fluorescence-based detection assays.
The antibody is affinity-purified using immunoglobulin-specific affinity chromatography, ensuring high specificity and low background noise. It is formulated in a stabilizing buffer to enhance performance and provide long-term stability.
Key Features & Benefits
✔ Highly Specific – Binds specifically to rabbit IgG (H+L) with minimal cross-reactivity.
✔ HRP Conjugation for Chemiluminescent/Colorimetric Detection – Compatible with ECL and TMB substrates.
✔ Versatile Detection Use – Ideal for Western blotting, ELISA, and IHC applications.
✔ High Sensitivity & Low Background – Affinity-purified for superior specificity.
✔ Stable Liquid Formulation – Stored in PBS with proprietary stabilizers for long-term stability.
✔ Wide Dilution Range – Recommended 1:500 - 1:5,000 depending on application.
Applications & Recommended Dilutions
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | 1:500 - 1:5,000 |
| Immunohistochemistry (IHC) | 1:6 - 1:60 |
| ELISA (ELISA) | 1:60 - 1:500 |
✔ Compatible with chemiluminescent (ECL), colorimetric (TMB, DAB), and fluorogenic substrates for visualization in WB, ELISA, and IHC.
✔ Western blot working dilutions for different HRP substrates:
- SuperSignal West Pico Substrate: 1:250 - 1:1,250
- SuperSignal West Dura Substrate: 1:600 - 1:3,000
- SuperSignal West Femto Substrate: 1:1,250 - 1:6,000
- ECL Western Blotting Substrate: 1:20 - 1:125
Product Specifications
| Feature | Details |
|---|---|
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Reactivity | Rabbit IgG (H+L) |
| Conjugate | Horseradish Peroxidase (HRP) |
| Form | Liquid |
| Concentration | 0.01 mg/mL |
| Immunogen | Purified Rabbit IgG, whole molecule |
| Storage Buffer | PBS with proprietary stabilizer (no preservative) |
| Purification Method | Affinity Purified |
| Storage Temperature | 4°C (Do NOT freeze) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| Regulatory Status | For Research Use Only (RUO) |
Target Information
The Goat anti-Rabbit IgG (H+L) Secondary Antibody binds specifically to the heavy and light chains of rabbit IgG, making it an essential detection reagent for a variety of immunoassays.
- Western Blot (WB): Detects rabbit primary antibodies in protein analysis studies.
- Immunohistochemistry (IHC): Used in tissue staining applications for detecting rabbit IgG primary antibodies.
- ELISA: Facilitates antibody-based quantification of proteins and antigens.
Storage & Stability
📦 Storage Temperature: 4°C (Refrigerated) upon receipt.
❄ Do NOT freeze, as this may affect antibody activity.
⚠ Shelf Life: Long-term stability when stored under recommended conditions.
Safety & Handling
⚠ For Research Use Only (RUO) – Not intended for human or clinical applications.
⚠ Wear PPE – Gloves, lab coat, and eye protection recommended.
⚠ Dispose of waste properly according to laboratory safety protocols.
Related Products & Alternative Secondary Antibodies
| Catalog # | Product Name | Conjugate | Unit Size |
|---|---|---|---|
| 31458 | Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody | HRP | 500 µL |
| A11008 | Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody | Alexa Fluor™ 488 | 1 mg |
| A11070 | F(ab')2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody | Alexa Fluor™ 488 | 500 µg |
| A21038 | Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody | Alexa Fluor™ 700 | 1 mg |
| A11046 | Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody | Alexa Fluor™ 350 | 1 mg |
| A32731 | Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody | Alexa Fluor™ Plus 488 | 1 mg |
| A21207 | Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody | Alexa Fluor™ 594 | 1 mg |
The Invitrogen™ Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP (Catalog # 32460) is a highly specific, HRP-conjugated secondary antibody designed for Western blot, ELISA, and IHC applications. Its strong HRP conjugation, high specificity, and optimized buffer formulation ensure low background noise and high signal intensity, making it a reliable choice for protein detection assays.