0
Thermo Scientific™ Proteinase K Powder (≥30 U/mg), 100mg
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | molecular biology | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | lyophilized powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Pierce™ Proteinase K is a highly active, broad-spectrum serine protease that hydrolyzes a wide range of peptide bonds, particularly at the carboxyl side of aliphatic, aromatic, or hydrophobic amino acids. It is an essential enzyme for nucleic acid purification workflows, especially when eliminating contaminating DNases and RNases.
Sourced from the fungus Tritirachium album, this subtilisin-like protease exhibits excellent stability across a wide range of pH and temperature conditions. Its activity is further enhanced at elevated temperatures (up to 65°C), making it ideal for rapid and efficient protein digestion.
Key Features & Benefits
-
High Specific Activity (≥30 U/mg)
– Enables fast and effective protein digestion, suitable for nucleic acid extraction. -
DNase and RNase Removal
– Commonly used to degrade nucleases in DNA/RNA purification protocols. -
Thermally Stable
– Active at elevated temperatures; activity increases up to 65°C. -
Chelator-Compatible
– Remains active in the presence of EDTA and other metal ion chelators, allowing simultaneous inactivation of calcium-dependent nucleases. -
Versatile Use
– Applicable in genomic DNA, plasmid DNA, total RNA extraction, and protein contamination removal.
Applications
- Genomic DNA isolation from tissues, blood, or cell cultures
- RNA purification in workflows requiring DNase/RNase removal
- Plasmid DNA purification
- Protein digestion in molecular biology and forensics
- Sample pre-treatment for PCR, qPCR, or sequencing workflows
Specifications
| Parameter | Details |
|---|---|
| Enzyme Type | Serine protease (subtilisin family) |
| Source Organism | Tritirachium album |
| Activity | ≥30 units/mg |
| Cleavage Specificity | Carboxyl side of aliphatic/aromatic/hydrophobic residues |
| Temperature Range | Optimal activity up to 65°C |
| pH Stability | Broad range (pH 4–12) |
| Chelator Compatibility | Active in presence of EDTA |
| Form | Lyophilized powder |
| Quantity | 100 mg |
Storage & Handling
- Store in the original container, protected from moisture and light.
- Do not store above –20°C.
- Reconstitute in buffer as required before use.
Thermo Scientific™ Pierce™ Proteinase K Powder is a reliable, high-activity enzyme for molecular biology applications requiring protein removal or nuclease inactivation. Its stability in challenging conditions and compatibility with chelating agents make it ideal for high-purity nucleic acid extraction and enzyme-based sample preparation.