Thermo Scientific™ Lysozyme Solution (50 mg/mL), 0.5 mL

The Thermo Scientific™ Lysozyme Solution is a high-performance enzyme solution designed to efficiently lyse bacterial cell walls, enhancing protein and nucleic acid extraction. Provided in a convenient liquid form, this enzyme solution ensures reliable and reproducible results across a range of molecular biology and biochemical applications.

Key Features:

1. High Efficiency:
   • Concentration: 50 mg/mL, delivering ≥50 units/mL activity at 5 μg/mL.
   • Significantly improves the extraction of proteins and nucleic acids from bacterial cells.
2. Enzymatic Activity:
   • Hydrolyzes 1,4-β-linkages in bacterial peptidoglycan, targeting N-acetylmuramic acid and N-acetyl-D-glucosamine residues.
   • Effective against Gram-positive bacteria; facilitates lysis of Gram-negative bacteria (e.g., E. coli, Salmonella, Shigella) when combined with nucleases like DNase I.
3. Convenient Liquid Form:
   • Ready-to-use glycerol freezer stock simplifies experimental workflows.
   • Clear and colorless solution, free of insoluble material for consistent handling.
4. Compatible with Downstream Applications:
   • Works seamlessly with Thermo Scientific Pierce Cell Lysis Reagents.
   • Enhances protein extraction for inclusion body purification and other molecular biology workflows.
5. Stable and Reliable:
   • pH: 4.7 ± 0.2, ensuring enzymatic stability.
   • Extinction coefficient: ~26.4 at 280 nm.
   • Isoelectric point: pH 11.0, enabling compatibility across a wide range of experimental conditions.

Applications:

1. Protein Extraction:
   • Efficiently extracts proteins from bacterial cells for research and biotechnological studies.
   • Aids in inclusion body purification by digesting cell debris.
2. Nucleic Acid Extraction:
   • Facilitates the release of high-quality DNA and RNA from bacterial cells, ideal for genomic and transcriptomic studies.
3. Cell Lysis for Downstream Analysis:
   • Prepares lysates for SDS-PAGE, mass spectrometry, and other protein analysis workflows.
4. Research on Peptidoglycan Hydrolysis:
   • Investigates bacterial cell wall structures and enzymatic interactions with peptidoglycan.

Specifications:

Unit Definition:

One unit is the amount of enzyme required to catalyze a decrease in absorbance at 450 nm of 0.001/min at 25°C, pH 6.24, in a 1 cm cuvette using a ~0.25 mg/mL suspension of Micrococcus luteus.

Advantages:

1. Enhanced Efficiency:
   • Breaks down bacterial cell walls for improved extraction of biomolecules.
   • Allows rapid lysis of bacterial cells, minimizing sample handling time.
2. Reliable Results:
   • Highly active and stable, ensuring consistent performance in molecular biology workflows.
3. Ready-to-Use:
   • Pre-formulated liquid enzyme eliminates preparation steps, saving time and reducing variability.
4. Versatile Application:
   • Suitable for a broad range of bacterial lysis requirements, including genomic studies and protein isolation.

Usage Instructions:

1. Thaw the enzyme solution on ice.
2. Add the appropriate volume of lysozyme solution to the bacterial suspension based on experimental requirements.
3. Incubate at the desired temperature and pH for lysis.
4. Proceed with downstream applications, such as protein purification or nucleic acid isolation.

Related Products:

• Thermo Scientific Pierce Cell Lysis Reagents for enhanced compatibility.
• DNase I Enzyme to complement lysozyme for Gram-negative bacterial lysis.

The Thermo Scientific™ Lysozyme Solution (50 mg/mL) is a powerful, reliable, and ready-to-use enzyme for bacterial lysis, making it an indispensable tool for protein and nucleic acid extraction workflows in molecular biology and biochemistry research.