Invitrogen™ TRIzol™ Plus RNA Purification Kit, 50 preps

The TRIzol™ Plus RNA Purification Kit provides a robust, reliable, and efficient method for isolating high-quality total RNA from diverse biological samples. Combining the powerful lysis capabilities of TRIzol™ Reagent with the effective silica cartridge-based purification of the PureLink™ RNA Mini Kit, this system delivers ultrapure RNA in about an hour, even from challenging sample types such as fibrous or fatty tissues.

Key Features:

1. Dual Technology Integration:
   • Combines TRIzol™-based lysis for superior RNA integrity and inhibition of RNase activity with PureLink™ spin column-based purification for efficient contaminant removal and RNA recovery.
2. Versatile Application Range:
   • Compatible with various sample types, including:
     • Animal cells and tissues.
     • Plant cells and tissues.
     • Bacteria and yeast.
3. Optimized for Gene Expression Studies:
   • Purifies RNA for downstream applications, including RT-PCR, qPCR, cDNA library construction, microarray analysis, NGS, and more.
4. High Yield and Integrity:
   • Yields up to 350 μg of total RNA with minimal degradation, even from challenging sample types.
5. Efficient Protocol:
   • Simplified workflow from TRIzol™ lysis to spin-column purification ensures consistent results within approximately 1 hour.

Specifications:

• Technology: Silica Spin Column + Organic Extraction.
• Purification Time: 1 hour.
• Sample Compatibility: Bacteria, cells, tissues, plants, yeast.
• Final Product: Total RNA.
• Starting Material:
  • Bacteria: ≤ 10⁷ cells.
  • Mammalian cells: ≤ 10⁷ cells.
  • Tissue: ≤ 100 mg.
  • Plant: ≤ 100 mg or 10⁷ cells.
  • Yeast: ≤ 10⁷ cells.
• Elution Volume: 30–300 μL.
• Yield: Up to 350 μg.
• Scale: Mini-prep.
• Unit Size: 50 preps.
• Compatibility: Manual, not high-throughput.

Contents & Storage:

All components are shipped and stored at room temperature for ease of use.

• TRIzol™ Reagent: 100 mL.
• Lysis Buffer: 125 mL.
• Wash Buffer I: 50 mL.
• Wash Buffer II: 15 mL.
• RNase-Free Water: 15.5 mL.
• Spin Cartridges with Collection Tubes: 50.
• Recovery Tubes: 50.

Applications:

The purified RNA is suitable for:

• RT-PCR and qPCR.
• cDNA library construction.
• Next-Generation Sequencing (NGS).
• Microarray analysis.
• Northern blotting and blot hybridization.
• Nuclease protection assays.
• In vitro translation.
• Nucleic acid labeling.

Advantages:

1. High Purity: TRIzol™ maintains RNA integrity by inhibiting RNases during lysis, while PureLink™ ensures contaminant-free purification.
2. Broad Compatibility: Effective across multiple species and sample types.
3. Quick and Reliable: Streamlined process delivers consistent results within an hour.
4. Convenient Storage: Components are stable at room temperature, making it easy to handle and store.

Ordering Information:

• Catalog Number:
  • 50 preps kit.
• Shipping Conditions: Room temperature.
• Storage: Room temperature for all reagents.

The TRIzol™ Plus RNA Purification Kit and the Direct-zol™ RNA Miniprep Kits are both designed for efficient RNA extraction from various biological samples. Below is a detailed comparison of their features:

• TRIzol™ Plus RNA Purification Kit: Suitable for applications requiring higher RNA yields and compatibility with a broader range of sample types.
• Direct-zol™ RNA Miniprep Kits: Ideal for rapid, user-friendly workflows with effective small RNA recovery.