0
Thermo Scientific™ Chloroform : isoamyl alcohol (24:1) (v/v) for molecular biology DNase-, RNase-, protease-free
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | Nucleic Acid Extraction | ||
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Chemical | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | Formula | CHCl₃,C₅H₁₂O |
Thermo Scientific's Chloroform:Isoamyl Alcohol (24:1) is a high-purity reagent designed for molecular biology applications. The mixture is DNase-, RNase-, and protease-free, ensuring minimal contamination and consistent results in sensitive workflows such as nucleic acid purification.
Key Features
- High Purity:
- Mixture contains ≥99.0% isoamyl alcohol as verified by gas chromatography (GC).
- Free from DNase, RNase, and protease activity, making it suitable for molecular biology applications.
- Optimal Ratio:
- Formulated in a 24:1 (v/v) ratio for efficient phase separation during nucleic acid extraction.
- Molecular Biology Grade:
- Specifically manufactured and tested for use in DNA and RNA workflows to ensure reliable results.
Specifications
| Property | Details |
|---|---|
| Chemical Formula | CHCl₃, C₅H₁₂O |
| Melting Point | –63 °C |
| Storage Temperature | Ambient |
| Appearance | Clear solution |
| GC Purity (Isoamyl Alcohol) | ≥99.0% |
| DNase, RNase, Protease Activity | None detected |
| MDL Number | MFCD00171265 |
| CAS Number | 67-66-3 |
| UN Number | UN 1888 |
| ADR Classification | 6.1, III |
Applications
- Nucleic Acid Purification:
- Facilitates phase separation in phenol-chloroform extraction protocols for isolating DNA or RNA.
- Molecular Biology Workflows:
- Used to remove proteins and other contaminants during nucleic acid preparation.
- General Laboratory Use:
- Ideal for other workflows requiring high-purity chloroform-isoamyl alcohol mixtures.
Advantages
- Enzyme-Free Guarantee: DNase-, RNase-, and protease-free, ensuring no degradation of nucleic acids.
- Reliable Purity: High GC purity of isoamyl alcohol for consistent phase separation.
- Ambient Storage: Stable under ambient conditions for ease of handling and storage.
Safety Information
- Hazards: Classified as toxic with potential health risks; handle with care.
- Precautions:
- Use in a well-ventilated area or under a fume hood.
- Wear appropriate personal protective equipment (gloves, goggles, and lab coat).
- UN Classification: UN 1888, ADR 6.1, III (hazardous material; follow transport regulations).
Thermo Scientific Chloroform:Isoamyl Alcohol (24:1) is a trusted solution for molecular biology applications. Its high purity and enzyme-free certification make it ideal for DNA and RNA extraction, ensuring reproducibility and reliability in your experiments.