Gibco™ Phytohemagglutinin, M form (PHA-M), 10 mL

Gibco™ Phytohemagglutinin, M form (PHA-M) is a biological reagent derived from the red kidney bean, Phaseolus vulgaris. It is widely used in immunology and cell biology research to stimulate lymphocytes and induce blastogenesis in mammalian mononuclear cells. This solution is particularly effective at promoting cell division in peripheral blood lymphocytes, making it an essential tool for various cell-based assays, including T-cell activation and proliferation studies. The preparation is performance-tested at a 1.5% concentration, ensuring consistent and reliable results.

Key Features:

• Induces Blastogenesis:
  Phytohemagglutinin (PHA-M) is a potent mitogen known for its ability to induce blastogenesis (cell division) in various mammalian mononuclear cells, including peripheral blood lymphocytes, when used at concentrations of 1-2% v/v.
• High Performance and Quality:
  Each lot of Gibco™ Phytohemagglutinin is rigorously performance tested at 1.5% using peripheral blood lymphocytes from a normal adult donor cultured for 72 hours in Gibco™ RPMI 1640 Medium, supplemented with Fetal Bovine Serum (FBS), heparin, antibiotics, and L-glutamine. This ensures reliable results in immune cell proliferation studies.
• cGMP Manufacturing:
  Gibco™ Phytohemagglutinin is manufactured in a facility compliant with current Good Manufacturing Practices (cGMP). The manufacturing facility is FDA-registered as a medical device manufacturer and certified under ISO 13485 standards, ensuring that the product meets the highest industry standards for quality and safety.
• Sterile-Filtered:
  The product is sterile-filtered to ensure purity and to prevent microbial contamination, making it suitable for sensitive cell culture experiments.

Specifications:

• Cell Type:
  Suitable for use with mammalian cells, specifically in inducing cell proliferation in mononuclear cells like lymphocytes.
• Form:
  Liquid form, ready to use.
• Product Type:
  Phytohemagglutinin, M form (PHA-M) from Phaseolus vulgaris.
• Shelf Life:
  24 months from the date of manufacture, ensuring long-term usability when stored correctly.
• Sterility:
  Sterile-filtered to guarantee contamination-free use in cell culture applications.
• Quantity:
  10 mL per vial.
• Storage Conditions:
  Store at temperatures between -5°C to -20°C, and protect from light to maintain product integrity.
• Shipping Conditions:
  Shipped on dry ice to maintain stability during transport.

Applications:

• T-Cell Activation:
  PHA-M is commonly used in research focused on T-cell activation and proliferation, making it an invaluable tool in immunological studies.
• Lymphocyte Proliferation Assays:
  This reagent is used to stimulate lymphocyte proliferation in vitro, which is essential for immune cell assays, cell-mediated immunity studies, and drug testing.
• Cell Culture Research:
  PHA-M is also used for culturing various mammalian mononuclear cells, particularly in research involving immune responses or cell signaling pathways.

Important Notes:

• For Research Use Only:
  Gibco™ Phytohemagglutinin is intended exclusively for research use and is not for use in diagnostic procedures or human/animal therapeutic applications.

Ordering Information: