Gibco™ Penicillin-Streptomycin (5,000 U/mL), 100X, 100mL

The Penicillin-Streptomycin Solution is an antibiotic combination widely used in cell culture applications to prevent bacterial contamination. Its dual-action targets both Gram-positive and Gram-negative bacteria, ensuring a sterile and contamination-free environment in cell culture systems. The solution is ideal for mammalian and insect cell culture applications.

Key Features and Benefits

1. Composition:
   • Penicillin:
     • Concentration: 5000 units/mL.
     • Mechanism: Inhibits bacterial cell wall synthesis by disrupting peptidoglycan cross-linking and inducing autolytic enzymes.
     • Source: Derived from Penicillium fungi.
   • Streptomycin:
     • Concentration: 5000 µg/mL.
     • Mechanism: Binds to the 30S ribosomal subunit, inhibiting bacterial protein synthesis and causing cell death.
     • Source: Derived from Streptomyces griseus.
2. Applications:
   • Effective for preventing bacterial contamination in cell culture systems.
   • Ideal for mammalian and insect cell cultures.
   • Commonly used in basic and advanced research laboratories.
3. Advantages:
   • Broad-spectrum antimicrobial activity.
   • Ready-to-use liquid format simplifies preparation.
   • Compatible with a variety of basal media, including DMEM, RPMI, and MEM.
   • Suitable for combined use with antifungal agents for enhanced contamination control.
4. Specifications:
   • Concentration: 100X stock solution.
   • Sterility: Sterile-filtered.
   • Shelf Life: 12 months from the date of manufacture.
   • Form: Liquid.
   • Unit Size: 100 mL.
5. Storage and Stability:
   • Storage Temperature: -5°C to -20°C.
   • Shipping Condition: Dry ice.
   • Shelf Life: 12 months when stored under recommended conditions.

Recommended Use

1. Preparation:
   • Thaw completely and mix thoroughly before use.
   • Dilute to a final working concentration of 50-100 units/mL penicillin and 50-100 µg/mL streptomycin in the culture medium.
2. Applications:
   • Add directly to the culture media to prevent bacterial contamination in mammalian and insect cell cultures.
   • Suitable for primary and continuous cell lines.
3. Eukaryotic and Bacterial Selection:
   • Effective for contamination control.
   • Recommended for maintaining clean cultures in research and production environments.

Specifications Table

Mechanisms of Action

Storage Recommendations

Comparison with Similar Products

General References

1. Fleming, A. "The Discovery of Penicillin," British Medical Journal, 1929.
2. Schatz, A. et al. "Streptomycin: An Antibiotic Remedy," Science, 1944.
3. Current Good Manufacturing Practices (cGMP) guidelines for cell culture reagents.

This Penicillin-Streptomycin solution ensures optimal contamination control in cell culture environments, making it an indispensable reagent for researchers worldwide.