Thermo Scientific™ Pierce™ Alkaline Phosphatase, ~20 mg/mL

Thermo Scientific™ Pierce™ Alkaline Phosphatase (CIP) is a purified preparation of calf intestinal alkaline phosphatase supplied in a ready-to-use liquid form. This high-activity enzyme preparation is ideal for use in protein research, molecular biology, and immunoassays, especially for the removal of phosphate groups from nucleic acids or as a colorimetric enzyme reporter in ELISA or Western blot applications.

The enzyme is supplied in Tris buffer containing magnesium and zinc ions, along with 50% glycerol for stability and ease of storage. The product requires no dialysis prior to use and is provided at a high concentration for convenience in downstream conjugation or direct assay use.

Key Features & Benefits

• Highly Purified Enzyme
  – Free from interfering contaminants and ready for direct conjugation or assay use.
• High Enzymatic Activity
  – Typically greater than 1600 units/mg, ensuring strong performance in both dephosphorylation and detection assays.
• Stable Liquid Formulation
  – Supplied in 50% glycerol for long-term storage without loss of activity.
• Concentrated Preparation
  – ~20 mg/mL concentration provides flexibility for assay optimization or conjugation.
• Buffered for Performance
  – Supplied in 5 mM Tris, 5 mM MgCl₂, 0.1 mM ZnCl₂, pH ~7.0 to maintain enzyme stability and functionality.

Applications

• DNA Dephosphorylation
  – Removal of 5′-phosphate groups from DNA to prevent self-ligation in cloning workflows.
• Reporter Enzyme in ELISA & Blots
  – Acts as a colorimetric detection enzyme when conjugated to antibodies or probes; compatible with substrates like pNPP or BCIP/NBT.
• Immunohistochemistry (IHC)
  – Used as a label in ABC (avidin-biotin complex) systems to visualize antibody binding in tissue samples.
• In Situ Hybridization & Probe Detection
  – Labels nucleic acid probes for colorimetric detection.

Technical Specifications

Pierce™ Alkaline Phosphatase (CIP) from Thermo Scientific offers exceptional enzymatic activity and reliability for protein and nucleic acid assays. Its highly purified, concentrated, and stable formulation makes it an excellent choice for conjugation, dephosphorylation, or as a detection enzyme in ELISA, blotting, and histological techniques.