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Thermo Scientific™ Pierce™ Horseradish Peroxidase, Biotinylated
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Specifications:
| Application | Protein Biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Recombinant Protein | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Pierce™ Horseradish Peroxidase, Biotinylated (B-HRP), is a highly purified form of the enzyme HRP conjugated to biotin. This reagent is designed for use in avidin-biotin complex (ABC) detection systems, especially for immunohistochemistry (IHC), ELISA, blotting, and other enzyme-based signal amplification methods. The covalent attachment of biotin enables HRP to be tightly and specifically bound by avidin, streptavidin, or NeutrAvidin™ conjugates for enhanced signal development in colorimetric or chemiluminescent detection protocols.
Key Features & Benefits
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Robust Signal Amplification
– Utilizes biotin-avidin interaction to amplify HRP-based enzyme signals in IHC, ELISA, and Western blotting. -
High-Specificity Detection
– Conjugation of biotin to HRP allows for highly specific detection using streptavidin or avidin-linked detection systems. -
Flexible Application Use
– Ideal for ABC staining protocols, enabling strong and localized signal development in tissue samples and blots. -
Convenient Storage & Stability
– Supplied in a lyophilized format for long-term storage and consistent activity upon reconstitution.
Applications
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Immunohistochemistry (IHC)
– Use in ABC-based systems for high-sensitivity staining of tissue sections using biotinylated primary or secondary antibodies. -
ELISA (Enzyme-Linked Immunosorbent Assay)
– Pair with streptavidin-coated plates for sandwich or indirect ELISAs. -
Western & Dot Blotting
– For use in avidin- or streptavidin-based detection of biotinylated proteins or targets. -
In Situ Hybridization & Cell Staining
– As a reporter enzyme in biotin-labeled probe systems.
Specifications Table
| Property | Description |
|---|---|
| Product Name | Pierce™ Horseradish Peroxidase, Biotinylated |
| Catalog Number | 29139 |
| Quantity | 5 mg |
| Label Type | Biotin |
| Conjugate | Horseradish Peroxidase (HRP) |
| Form | Lyophilized solid |
| Storage Temperature | 2°C to 8°C |
| Application Compatibility | IHC, ELISA, Western blot, ABC staining systems |
| Shipping Condition | Shipped on cold packs |
Thermo Scientific™ Pierce™ Biotinylated Horseradish Peroxidase is a powerful reagent designed for sensitive and specific detection of biotin-labeled molecules in a variety of biological assays. With robust enzymatic activity and efficient biotin conjugation, it serves as an essential tool in signal amplification protocols where precision and consistency are paramount.