Thermo Scientific™ N,N,N',N'-Tetrakis-(2-pyridylmethyl)ethylenediamine

Thermo Scientific™ N,N,N',N'-Tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN) is a cell-permeable, high-affinity chelator of transition and heavy metals such as zinc (Zn²⁺), iron (Fe²⁺/Fe³⁺), and copper (Cu²⁺).

Due to its exceptional metal-binding selectivity, TPEN is widely used in cell biology, biochemistry, and neurochemistry to regulate or deplete intracellular metal ion concentrations without disrupting overall cell membrane integrity.

Its versatility and permeability make it a valuable tool in metal-dependent enzyme studies, oxidative stress assays, apoptosis signaling, and metalloprotein regulation research.

Key Features

• High Metal Affinity: Efficient chelation of divalent metal ions (Zn²⁺, Fe²⁺, Cu²⁺, Mn²⁺).
• Cell-Permeable: Enables intracellular chelation in live-cell experiments.
• Research-Grade Purity: ≥95% (as confirmed by supplier’s CoA).
• Reversible Chelator: Allows dynamic control of intracellular metal ion concentrations.
• Applications: Metal homeostasis, apoptosis signaling, and oxidative stress studies.
• Stable Powder Form: Supplied as a high-purity solid for ease of handling and reproducibility.
• Research Use Only (RUO): For laboratory applications; not for therapeutic use.

Specifications

Applications

• Metal Ion Chelation: Removes intracellular zinc, iron, and copper for metal-regulated pathway studies.
• Apoptosis Research: Induces caspase activation via zinc depletion.
• Oxidative Stress Studies: Modulates reactive oxygen species (ROS) through metal sequestration.
• Neurobiology: Investigates metal ion effects in neuronal signaling and neurotoxicity.
• Enzyme Studies: Inhibition of metal-dependent enzymes for mechanistic analysis.
• Metalloprotein Regulation: Used to modulate metalloprotein activity in vitro and in vivo.

Safety & Handling

• Signal Word: Warning
• Hazard Statements:
  • H315 – Causes skin irritation
  • H319 – Causes serious eye irritation
  • H335 – May cause respiratory irritation
• Precautionary Statements:
  • P280 – Wear protective gloves/protective clothing/eye protection/face protection
  • P304 + P340 – If inhaled: Remove person to fresh air and keep comfortable
  • P302 + P352 – If on skin: Wash with plenty of soap and water
  • P312 – Call a POISON CENTER or doctor if you feel unwell
• Classification: Skin Irritation Cat. 2 | Eye Irritation Cat. 2 | STOT (Single Exposure) Cat. 3

The Thermo Scientific™ N,N,N',N'-Tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN) is a research-grade, high-affinity heavy metal chelator ideal for studying metal-dependent cellular processes, including apoptosis, oxidative stress, and metalloprotein regulation.

With high purity and cell permeability, it is a dependable reagent for precise control of intracellular metal ion concentrations in cellular, biochemical, and molecular biology research.