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Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit
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Specifications:
| Application | PCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit is a high-performance reverse transcription system designed for first-strand cDNA synthesis from RNA templates. It features the Maxima H Minus Reverse Transcriptase, a highly thermostable enzyme derived from MMLV RT via in vitro evolution. This enzyme is RNase H-deficient, allowing for synthesis of long, full-length cDNAs up to 20 kb, even from complex or GC-rich templates.
The kit is available in two configurations:
- Standard version (K1651 & K1652)
- With dsDNase (K1681 & K1682) – for integrated genomic DNA removal in a simplified one-tube workflow.
Key Features & Benefits
✅ High Efficiency & Thermostability
- Synthesizes full-length cDNA from RNA templates up to 20 kb
- Operates efficiently at 42°C to 65°C to overcome secondary RNA structures
- Reaction completed in just 30 minutes
✅ No RNase H Activity
- RNase H-minus (H–) reverse transcriptase ensures longer, intact cDNA synthesis by preventing degradation of RNA templates during extension.
✅ Flexible Priming Options
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Compatible with:
- Oligo(dT)18 primers (selective for mRNA)
- Random hexamer primers (for total RNA)
- Gene-specific primers (for targeted RT)
✅ Optional dsDNase Integration (K1681/K1682)
- Removes contaminating genomic DNA from RNA in 2 minutes, using a highly specific double-stranded DNase
- Enables one-tube gDNA removal + cDNA synthesis workflow
✅ Complete Kit with Quality Reagents
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Includes:
- Maxima H Minus Enzyme Mix (RT + RiboLock RNase Inhibitor)
- Oligo(dT)18 and Random Hexamer Primers
- 5X RT Buffer
- dNTP Mix
- Nuclease-free water
- dsDNase & 10X dsDNase buffer (in dsDNase versions)
Ordering Options & Formats
| Catalog Number | Contents | Reactions | dsDNase Included |
|---|---|---|---|
| K1651 | Kit only | 20 Rxns | No |
| K1652 | Kit only | 100 Rxns | No |
| K1681 | Kit + dsDNase | 20 Rxns | Yes |
| K1682 | Kit + dsDNase | 100 Rxns | Yes |
Applications
- RT-PCR and qRT-PCR (Real-Time PCR)
- cDNA Library Construction
- Gene Expression Analysis
- Antisense RNA Synthesis
- Probe Generation for Hybridization
Specifications
| Parameter | Details |
|---|---|
| Enzyme | Maxima H Minus Reverse Transcriptase |
| RNase H Activity | Absent |
| Reverse Transcription Temp. | 42°C–65°C |
| Reaction Time | ~30 min |
| Max cDNA Size | Up to 20 kb |
| GC-Rich Performance | High |
| Starting Material | Total RNA or mRNA |
| Primers Included | Oligo(dT)18, Random Hexamer |
| RNase Inhibitor | RiboLock (included in Enzyme Mix) |
| dNTP Mix | 10 mM (each of dATP, dTTP, dCTP, dGTP) |
| Storage Temperature | –20°C |
| Shipping Condition | Dry Ice |
| Output Format | First-Strand cDNA |
| Reagent Format | Separate components |
| Instrument Compatibility | qPCR cyclers, thermal cyclers |
Why Choose Maxima H Minus First Strand cDNA Synthesis Kit?
✅ High yields of full-length cDNA even from long or structured RNA templates
✅ Flexible priming strategies for total RNA or mRNA
✅ One-tube workflow with genomic DNA removal (dsDNase version)
✅ Fast reaction time—30 minutes to cDNA
✅ Proven performance in qPCR, RT-PCR, and gene expression analysis
Whether you need a robust reverse transcription system for routine use or demanding applications, the Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit provides superior performance, convenience, and flexibility.
- Pack Size: 20 Rxns, Kit only 100 Rxns, Kit only 20 Rxns Kit with dsDNase 100 Rxns Kit with dsDNase