Thermo Scientific™ 4-Methylumbelliferone ≥97%

4-Methylumbelliferone (also known as hymecromone) is a fluorogenic substrate and coumarin derivative commonly used in biochemical assays, particularly for the detection of enzymatic activity such as β-glucuronidase, lipase, and glycosidase. With high fluorescence when excited at ~360 nm and emission at ~450 nm, it serves as a sensitive tool in fluorometric detection systems.

This compound is also utilized in clinical diagnostics, cell biology, and pharmacological research for its utility in enzyme substrate studies, inhibitor screening, and drug delivery assessments. Supplied at ≥97% purity, it appears as a white to light yellow crystalline powder and is stable under ambient storage conditions.

Key Features

• High purity: ≥97% (HPLC ≥89%)
• Fluorescent coumarin derivative
• Sensitive substrate for fluorometric enzyme assays
• Stable and easy to handle solid powder form
• Excitation/emission: ~360 nm / ~450 nm
• GHS07 (Irritant) – handle with PPE
• For research use only

Applications

• β-glucuronidase activity assays
• Detection of glycosidases, lipases, phosphatases
• Fluorometric substrate in biochemical research
• Inhibitor screening in drug discovery
• Fluorescent labeling and tracking in cell biology
• Used in clinical diagnostics and high-throughput screening

Chemical & Physical Properties

Available Packaging

Thermo Scientific™ 4-Methylumbelliferone, ≥97% is a highly versatile fluorogenic reagent ideal for enzyme assays, biochemical analysis, and fluorescence-based detection systems. With reliable purity and strong fluorescence performance, it is an essential compound for clinical research, diagnostics, and pharmaceutical studies.