Thermo Scientific™ 4-Methylumbelliferone ≥97%

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Formula: C₁₀H₈O₃
Storage Temperature: Ambient
CAS Number: 90-33-5

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Specifications:
Application molecular biology, Fluorescence-Based Assay
Storage Temperature Ambient
Product Type Laboratory Chemical, Enzymes and Substrates Forms Powder
Product Brand Thermo Fisher Scientific™, thermo Scientific
Product Grade Analytical grade Formula C₁₀H₈O₃

4-Methylumbelliferone (also known as hymecromone) is a fluorogenic substrate and coumarin derivative commonly used in biochemical assays, particularly for the detection of enzymatic activity such as β-glucuronidase, lipase, and glycosidase. With high fluorescence when excited at ~360 nm and emission at ~450 nm, it serves as a sensitive tool in fluorometric detection systems.

This compound is also utilized in clinical diagnostics, cell biology, and pharmacological research for its utility in enzyme substrate studies, inhibitor screening, and drug delivery assessments. Supplied at ≥97% purity, it appears as a white to light yellow crystalline powder and is stable under ambient storage conditions.

Key Features

  • High purity: ≥97% (HPLC ≥89%)
  • Fluorescent coumarin derivative
  • Sensitive substrate for fluorometric enzyme assays
  • Stable and easy to handle solid powder form
  • Excitation/emission: ~360 nm / ~450 nm
  • GHS07 (Irritant) – handle with PPE
  • For research use only

Applications

  • β-glucuronidase activity assays
  • Detection of glycosidases, lipases, phosphatases
  • Fluorometric substrate in biochemical research
  • Inhibitor screening in drug discovery
  • Fluorescent labeling and tracking in cell biology
  • Used in clinical diagnostics and high-throughput screening

Chemical & Physical Properties

PropertySpecification
Chemical Name4-Methylumbelliferone
CAS Number90-33-5
Molecular FormulaC₁₀H₈O₃
Molecular Weight176.17 g/mol
MDL NumberMFCD00006866
Merck Index14,4854
Melting Point186–191 °C
AppearanceWhite to off-white/light yellow powder
Water Content≤8%
HPLC Purity≥89.0%
Storage TemperatureAmbient
EINECS Number201-986-7
Hazard ClassificationGHS07 – Irritant

Available Packaging

Catalog Number
Pack TypePack Size
A10337.18
Glass bottle (solids)50 g
A10337.30
Glass bottle (solids)250 g
A10337.0B
Glass bottle (solids)1 kg


Thermo Scientific™ 4-Methylumbelliferone, ≥97% is a highly versatile fluorogenic reagent ideal for enzyme assays, biochemical analysis, and fluorescence-based detection systems. With reliable purity and strong fluorescence performance, it is an essential compound for clinical research, diagnostics, and pharmaceutical studies.

  • Pack Size:   250g 1kg 50g
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