SolisFAST® 1-step RT-PCR Kit with UNG Ready to Load

The SolisFAST® 1-step RT-PCR Kit with UNG Ready to Load provides a fast, sensitive, and contamination-resistant solution for RNA analysis, combining reverse transcription and PCR in a single-tube workflow. This streamlined approach minimizes pipetting steps, reduces variability between reactions, and shortens the time from setup to results. The Ready-to-Load variant simplifies post-PCR handling by incorporating inert tracking dyes, allowing samples to be loaded directly onto agarose gels without additional preparation.

This kit delivers end-point RT-PCR results in under 1 hour, with sensitivity down to 0.1 pg of RNA. It supports multiplex amplification of up to 5 RNA targets, making it ideal for studies requiring efficiency and reproducibility.

Key Features & Benefits

• One-Tube Workflow – Reverse transcription and PCR amplification in a single reaction, reducing contamination risk and hands-on time.
• Fast Protocols – Obtain RT-PCR results in less than 60 minutes, improving turnaround time for critical workflows.
• High Sensitivity – Detects as little as 0.1 pg RNA, enabling accurate analysis of low-abundance transcripts.
• Multiplexing – Supports amplification of up to 5 targets simultaneously, suitable for complex studies.
• Carryover Contamination Prevention – Preblended Salini UNG® enzyme and dUTPs prevent false positives.
• AmpliBoost™ RT-PCR Enhancer – Improves performance with GC-rich templates and RNA with challenging secondary structures.
• Ready-to-Load Convenience – Built-in inert loading dyes eliminate gel loading steps, saving time and simplifying analysis.
• Enzyme Stability – Proprietary Stability TAG™ technology ensures robustness and tolerance to room temperature handling.

Applications

• Gene Expression Analysis – Reliable detection of RNA transcript levels.
• Virus Detection – Identification of RNA viruses in research workflows.
• Presence/Absence Testing – Confirming RNA template integrity and availability.
• Cloning Workflows – Amplification of RNA templates up to 2 kb for downstream cloning.
• Singleplex and Multiplex RT-PCR – Ideal for both simple and complex end-point assays.

Kit Components

• 4× One-step HOT SolisFAST® Mix with UNG Ready to Load – Includes polymerase, dUTPs, Salini UNG®, optimized reaction buffer, inert dyes, and MgCl₂.
• 40× One-step RT Mix – Contains thermostable SOLIScript® and FIREScript® reverse transcriptases, plus RiboGrip® RNase Inhibitor.
• 5× AmpliBoost™ RT-PCR Enhancer – Improves sensitivity and amplifies GC-rich targets.
• Nuclease-Free Water.
• Green Loading Dye – Dual dye system (blue & yellow) for electrophoresis tracking.

Product Specifications

Ordering Information

The SolisFAST® 1-step RT-PCR Kit with UNG Ready to Load combines speed, sensitivity, and convenience for RNA analysis in a single kit. With its integrated reverse transcriptases, contamination control system, AmpliBoost™ enhancer, and direct gel loading capability, it is ideally suited for gene expression studies, viral detection, multiplex assays, and cloning workflows where accuracy and time efficiency are critical.