Liofilchem™ MTS™ Temocillin TMO 0.064-1024, MIC Test strips

The Liofilchem™ MTS™ Temocillin TMO MIC Test Strips (0.064–1024 µg/mL) are precision diagnostic tools designed to determine the Minimum Inhibitory Concentration (MIC) of Temocillin, a narrow-spectrum beta-lactam antibiotic. Temocillin is specifically effective against beta-lactamase-producing Gram-negative pathogens, including AmpC, ESBL, and carbapenem-resistant Enterobacterales.

Key Features

1. Antibiotic Spectrum:
   • Active against multidrug-resistant Escherichia coli, Klebsiella pneumoniae, and other Enterobacterales.
   • Beta-lactamase stability makes it effective for strains resistant to other beta-lactams.
2. MIC Range:
   • Covers a broad range from 0.064 µg/mL to 1024 µg/mL, suitable for susceptibility testing in clinical and research settings.
3. Standard Compliance:
   • Follows EUCAST and CLSI guidelines for MIC determination.
   • CE-marked and IVD (In Vitro Diagnostic) certified.
4. Applications:
   • Detection of resistance mechanisms in Gram-negative bacteria, including AmpC and ESBL producers.
   • Differentiation of carbapenemase-producing Enterobacterales (CPE).
5. Storage Conditions:
   • Short-term storage: 2–8°C.
   • Long-term storage: -20°C.

Testing Procedure

1. Preparation:
   • Prepare a bacterial suspension equivalent to a 0.5 McFarland standard using colonies from a fresh agar plate.
   • Inoculate a Mueller-Hinton Agar (MHA) plate by evenly spreading the suspension with a sterile swab.
2. Application of Test Strips:
   • Place the Liofilchem™ MTS™ Temocillin TMO strip on the agar surface using sterile forceps or an applicator.
   • Ensure the strip adheres completely to the agar.
3. Incubation:
   • Incubate the plate at 35 ± 2°C for 16–20 hours in ambient air.
4. Interpretation of Results:
   • Read the MIC value where the inhibition ellipse intersects the scale on the strip.
   • Compare the MIC to EUCAST or CLSI breakpoints for clinical interpretation.

Quality Control

EUCAST and CLSI Breakpoints

Reference Data

• EUCAST Clinical Breakpoint Tables: Version 12.0 (2022).
• CLSI M100 Standards: Performance Standards for Antimicrobial Susceptibility Testing.
• Peer-reviewed studies demonstrating high reliability of Liofilchem™ MTS™ for Enterobacterales resistance profiling.

Regulatory and Certification Data

• IVD Certified: Meets European In Vitro Diagnostic Regulation (IVDR) requirements.
• CE Marked: Conforms to EU safety and performance standards.
• ISO 9001 and ISO 13485 Compliance: Manufactured under stringent quality control protocols.

Comparisons with Other Brands

The Liofilchem™ MTS™ Temocillin TMO 0.064–1024 MIC Test Strips provide precise and reliable MIC determination for clinical and research laboratories. With its broad range, regulatory compliance, and ease of use, it is an indispensable tool for monitoring beta-lactam resistance, particularly in multidrug-resistant Enterobacterales.