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Invitrogen™ LIVE/DEAD™ Fixable Dead Cell Stain Sampler Kit
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | -20°C | ||
| Product Type | Flow Cytometry Reagents | Forms | Lyophilized |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Multiplex-Ready Viability Dye Panel for Flow Cytometry – Fixation-Compatible and Laser-Compatible
The LIVE/DEAD™ Fixable Dead Cell Stain Sampler Kit is an all-in-one solution for discriminating live and dead cells in flow cytometry, especially for workflows requiring fixation and permeabilization. The kit includes eight spectrally distinct, amine-reactive viability dyes compatible with UV, violet, blue, and red laser lines, enabling flexible integration into multicolor flow cytometry panels.
These dyes covalently bind to amines on both the surface and interior of dead cells, providing a high-contrast, bright signal. The staining pattern is preserved after fixation, making this kit ideal for viability gating in intracellular staining workflows.
Key Features and Benefits
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Eight Color Options in One Kit
Includes 8 x 40-test vials of LIVE/DEAD™ Fixable Dyes:- Blue, Aqua, Violet, Yellow (UV/violet laser)
- Green, Red (blue laser)
- Far Red, Near-IR (red laser)
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Fixation-Compatible
Amine-reactive dyes retain signal after formaldehyde fixation and saponin-based permeabilization. -
Bright and Distinct Signal
Provides a >50-fold difference in fluorescence between live and dead cells, enabling clean viability gating. -
Flexible Panel Design
Designed for use with single-channel gating, preserving channels for other functional or surface markers. -
Stable Lyophilized Format
Each dye is freeze-dried to ensure long-term stability. Includes 500 µL DMSO for reconstitution.
Applications
- Live/dead discrimination in flow cytometry
- Viability gating prior to intracellular cytokine or transcription factor staining
- Optimization of multicolor panels with viability controls
- Dead cell exclusion in fixed and permeabilized samples
- Panel design testing for immune profiling and functional assays
Technical Specifications
| Property | Details |
|---|---|
| Product Name | LIVE/DEAD™ Fixable Dead Cell Stain Sampler Kit |
| Catalog Number | L34960 |
| Laser Compatibility | UV, Violet, Blue, Red |
| Excitation (range) | 350–750 nm (varies by dye) |
| Emission (range) | 450–775 nm (varies by dye) |
| Colors Included | Blue, Aqua, Violet, Yellow, Green, Red, Far Red, Near-IR |
| Form | Lyophilized dye, DMSO included |
| Format | 8 vials + 500 μL DMSO |
| Stain Type | Amine-reactive viability dyes |
| Detection Method | Fluorescence |
| Instrument Compatibility | Flow cytometer with multiple lasers |
| Cell Permeability | Impermeant |
| Storage Conditions | –20°C, protect from light |
| Shipping Conditions | Room temperature |
| Product Line | LIVE/DEAD™, Molecular Probes™ |
| Unit Size | Each (1 Kit) |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Kit Contents
- 8 x 40-test vials of LIVE/DEAD™ Fixable Dead Cell Stains (1 per color)
- 1 vial (500 µL) of DMSO for dye reconstitution
The LIVE/DEAD™ Fixable Dead Cell Stain Sampler Kit is a comprehensive and flexible solution for viability gating in flow cytometry. With eight distinct colors spanning the full laser spectrum, it enables precise dead cell exclusion and seamless integration into multicolor immunophenotyping panels, even following fixation.