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Invitrogen™ UltraComp eBeads™ Compensation Beads
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Flow Cytometry Reagents | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Universal Single-Color Compensation Beads for Mouse, Rat, and Hamster Antibodies in Multicolor Flow Cytometry
UltraComp eBeads™ Compensation Beads are high-performance, ready-to-use beads designed for use as single-color compensation controls in multicolor flow cytometry experiments. These beads bind fluorochrome-conjugated antibodies from mouse, rat, or hamster origin, regardless of immunoglobulin light chain, enabling standardized and reproducible compensation setup across a wide range of laser lines.
Each drop of UltraComp eBeads contains a positive and negative population, producing a bimodal fluorescence signal after antibody staining—ideal for defining compensation values across detectors in high-parameter panels.
Key Features and Benefits
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Species Flexibility
Binds mouse, rat, and hamster antibodies, regardless of light chain specificity. -
Compatible with All Laser Lines
Designed for use with fluorochromes excited by UV (355 nm), violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633–640 nm) lasers. -
Bimodal Fluorescence Signal
Each drop contains a positive and negative bead population, simplifying compensation setup by providing distinct signal separation. -
Universal Use Across Panels
Works with all fluorochrome-conjugated antibodies, including tandem dyes, making it suitable for complex flow cytometry experiments. -
Convenient and Stable Format
Provided in a dropper bottle format; stable and ready to use without additional preparation.
Applications
- Single-color compensation controls for multicolor flow cytometry
- Standardization of fluorescence compensation for mouse, rat, or hamster antibody panels
- Quality control in immune profiling, T/B cell assays, and intracellular staining workflows
Technical Specifications
| Property | Details |
|---|---|
| Product Name | UltraComp eBeads™ Compensation Beads |
| Catalog Numbers | 01-2222-41 (25 tests), 01-2222-42 (100 tests) |
| Species Reactivity | Mouse, Rat, Hamster |
| Laser Compatibility | UV (355 nm), Violet (405 nm), Blue (488 nm), Green (532 nm), Yellow-Green (561 nm), Red (633–640 nm) |
| Application | Flow Cytometric Compensation |
| Reagent Type | Compensation Beads |
| Product Format | Dropper bottle |
| Instrument Compatibility | Flow cytometer |
| Recommended Buffer | PBS or HBSS with protein (e.g., BSA or FBS) and sodium azide |
| Additive Compatibility | No surfactants or additional additives recommended |
| Storage Conditions | As per label instructions; room temperature unless otherwise stated |
| Product Line | eBeads™ |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Invitrogen™ UltraComp eBeads™ Compensation Beads offer a robust, flexible, and validated solution for standardizing single-color compensation controls in multicolor flow cytometry. Their compatibility with a wide range of fluorochromes and laser lines makes them an essential tool for any researcher conducting high-parameter immune profiling or complex antibody panel optimization.
- Pack Size: For 100 Tests for 25 Tests