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Invitrogen™ PureLink™ Quick Gel Extraction Kit
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Specifications:
| Application | Gel Electrophoresis, DNA Extraction and Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ Quick Gel Extraction Kit provides a rapid and efficient method for purifying DNA fragments from agarose gels prepared with TAE or TBE buffers. Using silica spin-column or vacuum-based technology, the kit enables reliable recovery of DNA fragments ranging from 40 bp to 10 kb within approximately 30 minutes.
Purified DNA is free from agarose, dyes, and proteins, ensuring high-quality results for a wide range of downstream molecular biology applications. With a binding capacity of up to 15 μg DNA per column, the kit delivers consistently high recovery while maintaining DNA integrity for sensitive workflows.
Key Features
- Flexible Gel Compatibility – Works with TAE- or TBE-based agarose gels of varying melting points.
- Broad Fragment Range – Purifies DNA fragments between 40 bp and 10 kb.
- Fast & Efficient – DNA extraction completed in ~30 minutes.
- High Capacity – Recovers up to 15 μg DNA per column.
- Versatile Technology – Silica-based columns can be used with centrifugation or vacuum.
- High Purity Output – Produces DNA suitable for PCR, sequencing, cloning, labeling, and in vitro transcription.
- Eco-Friendly – Sustainable packaging and reduced hazardous components.
Applications
- DNA recovery from agarose gels (linear fragments only).
- PCR amplicon recovery for cloning and sequencing.
- Preparation of DNA for restriction enzyme digestion and labeling.
- Purification of DNA for nucleic acid hybridization and Southern blotting.
- Sample preparation for in vitro transcription and other enzymatic assays.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Gel Extraction Kit |
| Catalog Numbers | K210012 (50 preps), K210025 (250 preps) |
| Technology | Silica spin-column or vacuum-based |
| Sample Type | Linear DNA fragments in agarose gel slices |
| Fragment Size Range | 40 bp – 10 kb |
| Binding Capacity | ≤15 μg DNA |
| Test Time | ~30 minutes |
| Elution Volume | 50 μL |
| Visualization | Compatible with ethidium bromide and SYBR™ Safe |
| Applications | PCR, sequencing, cloning, labeling, in vitro transcription |
| Storage Conditions | Columns at 2–8 °C recommended |
| Shipping Condition | Room temperature |
| Green Features | Less hazardous, sustainable packaging |
| Unit Size | Each kit (50 or 250 preps) |
Kit Components
- Gel Solubilization Buffer (L3)
- Wash Buffer (W1)
- Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
- Quick Gel Extraction Columns
- Wash Tubes and Recovery Tubes
Unique Advantages
- Purifies high-quality DNA suitable for the most demanding downstream applications.
- Eliminates contaminants such as agarose and dyes for improved reproducibility.
- Offers both centrifugation and vacuum formats for laboratory flexibility.
- Eco-conscious design reduces laboratory waste and hazards.
The Invitrogen™ PureLink™ Quick Gel Extraction Kit is a reliable and efficient solution for DNA recovery from agarose gels. With its broad fragment range, high yield, and compatibility with multiple downstream applications, it is an indispensable tool for molecular biology research. By combining convenience, performance, and sustainability, the kit ensures consistent and reproducible results in PCR, sequencing, cloning, and beyond.
- Pack Size: For 50 preps For 50 Preps