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Thermo Scientific™ GeneJET PCR Purification Kit
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Specifications:
| Application | PCR | ||
| Storage Temperature | Room Temperature | ||
| Product Type | PCR Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ GeneJET PCR Purification Kit offers a fast and reliable method for the purification of high-quality DNA fragments from PCR, restriction digestion, ligation, or other enzymatic reactions. Utilizing proprietary silica-based membrane technology in a convenient spin-column format, this kit enables efficient removal of primers, enzymes, nucleotides, and salts—eliminating the need for toxic phenol-chloroform extraction or labor-intensive resin procedures.
Capable of recovering DNA fragments ranging from 25 bp to 20 kb, this kit provides exceptional yield (up to 100%) and is optimized for downstream molecular biology applications such as sequencing, cloning, labeling, in vitro transcription, and more.
Key Features
- High recovery efficiency: 90–100% recovery for DNA fragments from 100 bp to 10 kb
- Wide size range: Purifies DNA fragments between 25 bp and 20 kb
- Fast protocol: Complete purification in as little as 5 minutes
- Maximum yield: Each column binds up to 25 μg of DNA
- Safe and reliable: No phenol, chloroform, or ethanol precipitation required
- Convenient: Spin columns come capped and preassembled with collection tubes
Applications
Ideal for rapid purification of double-stranded DNA (dsDNA) from a variety of enzymatic reactions, including:
- PCR amplification products
- Restriction enzyme digestion reactions
- Ligation reactions
- Labeling and fluorescent tagging
- In vitro transcription templates
- DNA sequencing and cloning workflows
- In situ hybridization
Specifications
| Property | Details |
|---|---|
| Format | Spin Column |
| Sample Type | dsDNA from PCR or enzymatic reactions |
| Binding Capacity | Up to 25 μg DNA per column |
| Fragment Size Range | 25 bp to 20 kb |
| Recovery Rate | 90–100% for 100 bp to 10 kb |
| Isolation Technology | Silica-based membrane |
| Processing Time | ≤5 minutes |
| High-throughput Compatibility | Manual (not high-throughput) |
| Downstream Applications | qPCR, sequencing, cloning, labeling, hybridization |
| Product Line | GeneJET™ |
| Storage Conditions | Store columns at 2–8 °C for optimal performance |
| Unit Size | Each kit (50 or 250 preps available) |
Available Formats
| Catalog No. | Kit Size |
|---|---|
| K0701 | 50 preps |
| K0702 | 250 preps |
Kit Components
- GeneJET Purification Columns (preassembled with collection tubes)
- Binding Buffer
- Wash Buffer (concentrated)
- Elution Buffer
- User Manual
The Thermo Scientific™ GeneJET PCR Purification Kit is a cost-effective and high-performance solution for rapid DNA purification in everyday molecular biology workflows. Whether preparing samples for cloning, sequencing, or transcription, this kit delivers reproducible, high-yield results with minimal hands-on time and maximum reliability.
- Pack Size: For 50 preps For 50 Preps