Invitrogen™ Power Blotter 1-Step™ Transfer Buffer (5X)

The Invitrogen™ Power Blotter 1-Step™ Transfer Buffer (5X) is a high-efficiency buffer optimized for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes. Designed for use with the Power Blotter System, this buffer enables fast and consistent protein transfer in just 5 to 12 minutes, significantly reducing blotting time while maintaining high transfer efficiency.

With a high ionic strength formulation, this buffer ensures efficient protein migration and is compatible with other semi-dry transfer devices equipped with a high-current power supply.

Key Features & Benefits

1. Fast & High-Efficiency Protein Transfer

• Transfers proteins in just 5–12 minutes, reducing workflow time.
• Maintains high transfer efficiency, comparable to traditional wet transfer methods.
• Enables fast, reproducible Western blot results.

2. Optimized for Power Blotter System

• Designed for seamless performance with Power Blotter Pre-cut Membranes and Filters.
• Ensures even protein transfer with high ionic strength buffer composition.
• Eliminates the need for expensive specialty consumables, making it cost-effective.

3. Compatible with Other Semi-Dry Transfer Devices

• Works with other semi-dry transfer systems using high-current power supplies (1.3–5.0 amps).
• Provides consistent, high-resolution protein transfers across different setups.
• Voltage limited to 25V, with constant current of ~22–23 mA/cm² ensuring uniform transfer.

4. Reliable Performance with Multiple Detection Methods

• Compatible with chemiluminescence, fluorescence, and chromogenic Western blot detection.
• Works with both nitrocellulose and PVDF membranes, offering flexibility for different experiments.
• Prevents excessive heat buildup, protecting sample integrity.

Specifications

Available Product Variants

Compatible Accessories & Consumables

Applications

1. Western Blotting & Protein Analysis

• Rapid protein transfer for immunodetection and proteomics research.
• Optimized for semi-dry Western blot workflows.
• Compatible with both nitrocellulose and PVDF membranes.

2. Molecular Biology & Biochemistry Research

• Designed for high-throughput Western blot workflows, improving research efficiency.
• Supports high-sensitivity detection using chemiluminescence, fluorescence, and staining methods.

3. Biotech & Pharmaceutical Research

• Used for protein expression studies, immunoblotting, and biotherapeutic research.
• High-throughput compatibility with Power Blotter Systems for large-scale experiments.

Storage & Handling

• Store at room temperature in a dry environment.
• Use at 5X concentration or dilute as needed for specific applications.
• Ensure proper buffer coverage in semi-dry transfer systems for optimal performance.

Ordering Information

The Invitrogen™ Power Blotter 1-Step™ Transfer Buffer (5X) is a high-performance, fast-acting buffer optimized for semi-dry Western blot protein transfer. When used with the Power Blotter System, it enables rapid, high-efficiency protein transfer in just 5–12 minutes while maintaining high-resolution blotting quality. This cost-effective, ready-to-use solution is compatible with both nitrocellulose and PVDF membranes, making it an essential reagent for researchers conducting Western blotting experiments.