Invitrogen™ Power Blotter Select Transfer Stacks, PVDF, 0.2 μm, regular size, 13 cm x 8.3 cm
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Specifications:
Application | Protein Electrophoresis & Western Blotting | ||
Storage Temperature | Room Temperature | ||
Product Type | Membranes | Forms | Kit with Various components |
Product Brand | Thermo Fisher Scientific™ | ||
Product Grade | Molecular Biology | ||
The Invitrogen™ Power Blotter Select Transfer Stacks, PVDF, Regular Size are pre-assembled, disposable transfer stacks designed for fast and high-efficiency Western blot protein transfer using the Power Blotter System. These stacks contain an integrated PVDF membrane, optimized for semi-dry blotting of proteins from polyacrylamide gels (SDS-PAGE), ensuring high binding capacity, uniform transfer, and increased detection sensitivity.
Each stack includes filter paper, a pre-activated PVDF membrane, and pre-incorporated cathode and anode buffers, ensuring rapid and reproducible protein transfer in just 7 minutes with minimal setup.
Key Features & Benefits
1. Pre-Assembled for Rapid & Reliable Protein Transfer
- Pre-assembled stacks eliminate buffer preparation, reducing manual handling.
- Semi-dry protein transfer is completed in as little as 7 minutes.
- Ensures high transfer efficiency with minimal protein loss.
2. High-Performance PVDF Membrane (0.2 μm Pore Size, Low Fluorescence)
- Higher binding capacity than nitrocellulose (240 μg/cm²), ideal for low-abundance proteins.
- Pre-activated and ready to use, eliminating the need for alcohol pretreatment.
- Compatible with staining, immunodetection, fluorescence, and radiolabeling detection methods.
- Hydrophobic interactions enhance protein retention, ensuring strong signal intensity and clarity.
3. Reliable & Reproducible Protein Transfer
- Uniform protein transfer across the membrane for consistent Western blot results.
- Minimizes background noise, enhancing signal-to-noise ratio in detection.
- Optimized for use with Power Blotter Systems, ensuring batch-to-batch consistency.
4. Regular-Size Format for Versatile Use
- Dimensions: 13 cm × 8.3 cm, compatible with:
- Invitrogen Novex and NuPAGE midi gels (or equivalent).
- Two mini-size gels (Novex, Bolt, and NuPAGE mini gels, or equivalent).
- Fully compatible with Power Blotter System and Power Blotter XL System.
- Ready-to-use format minimizes workflow errors and maximizes efficiency.
Specifications
Property | Details |
---|---|
Model | Power Blotter Select Transfer Stacks, PVDF, Regular Size |
Mode of Transfer | Semi-Dry |
Membrane Material | PVDF |
Pore Size | 0.2 μm |
Protein Binding Capacity | 240 μg/cm² |
Transfer Time | 7–12 minutes |
Compatible Gels | Novex, Bolt, NuPAGE midi and mini gels |
Dimensions (L × W) | 13 cm × 8.3 cm |
Shipping Condition | Room Temperature |
Sufficient For | 10 or 40 blots |
Unit Size | Each |
Available Product Variants
Catalog Number | Sufficient For |
---|---|
PB5310 | 10 Blots |
PB5340 | 40 Blots |
Compatible Accessories & Consumables
Product | Description | Quantity |
---|---|---|
Power Blotter Select Transfer Stacks, PVDF, Mini | Pre-cut PVDF transfer stacks for semi-dry blotting | 10 or 40 stacks |
Power Blotter Select Transfer Stacks, Nitrocellulose, Mini | Nitrocellulose membrane transfer stacks for mini gels | 10 or 40 stacks |
Power Blotter Select Transfer Stacks, Nitrocellulose, Regular Size | Larger nitrocellulose transfer stacks | 10 or 40 stacks |
Power Blotter 1-Step Transfer Buffer | Optimized buffer for semi-dry Western blotting | 1L |
Power Blotter Station | Integrated blotting system for protein transfer | 1 unit |
Applications
1. Western Blotting & Protein Analysis
- Rapid and reliable protein transfer for immunodetection and proteomics research.
- Optimized for PVDF-based Western blot detection methods.
- Enhances protein retention and signal clarity for fluorescence-based detection.
2. Molecular Biology & Biochemistry Research
- Designed for multi-gel transfer workflows, improving laboratory efficiency.
- Supports high-sensitivity detection using chemiluminescence, fluorescence, and staining methods.
3. Biotech & Pharmaceutical Research
- Used for protein expression studies, immunoblotting, and biotherapeutic research.
- High-throughput compatibility with Power Blotter Systems for large-scale experiments.
Storage & Handling
- Store at room temperature in a dry environment.
- Handle with gloves to prevent contamination of transfer membranes.
- Ensure proper alignment with gels for optimal transfer efficiency.
Ordering Information
Catalog Number | Description | Sufficient For |
---|---|---|
PB5310 | Power Blotter Select Transfer Stacks, PVDF, Regular Size | 10 Blots |
PB5340 | Power Blotter Select Transfer Stacks, PVDF, Regular Size | 40 Blots |
The Invitrogen™ Power Blotter Select Transfer Stacks, PVDF, Regular Size provide fast, efficient, and high-sensitivity semi-dry Western blot protein transfers. Designed for use with Power Blotter Systems, these pre-assembled transfer stacks streamline the blotting process, offering high-protein binding capacity and uniform transfer performance.
- Pack Size: Sufficient for 10 Blots Sufficient for 40 Blots