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Invitrogen™ Alignflow™ Flow Cytometry Alignment Beads, 3mL
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Flow Cytometry Reagents | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Reliable Calibration Beads for Laser Alignment, Focusing, and Optical Optimization in Flow Cytometry
Invitrogen™ AlignFlow™ Flow Cytometry Alignment Beads are high-quality, internally dyed fluorescent microspheres used to align, focus, and calibrate flow cytometers. These polystyrene beads are available in two sizes (2.5 µm and 6.0 µm) and are optimized for excitation by blue (488 nm), red (630–640 nm), or UV (350–370 nm) lasers. Their uniform size and intensity enable reliable and reproducible laser performance checks without consuming valuable biological samples.
AlignFlow™ beads are ideal for flow cytometry core labs and researchers requiring consistent performance across instruments and experiments.
Key Features and Benefits
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Laser-Specific Fluorescence Emission
- Blue Laser (488 nm): Emission 515–660 nm
- Red Laser (633–640 nm): Emission 645–680 nm
- UV Laser (350–370 nm): Emission 400–470 nm
- UV version is also excitable with violet lasers (405 nm)
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High Uniformity for Reliable Calibration
Very low forward scatter (FSC) CV and fluorescence intensity CV ensure consistent performance and repeatable instrument settings. -
Internally Stained for Stability
Fluorophores are embedded inside the bead core, protecting against environmental degradation and ensuring long-term signal stability. -
Dropper Vial Convenience
Each 3 mL kit includes 2 vials (1.5 mL each), allowing for easy dispensation and minimal waste. -
Broad Compatibility
Use with any flow cytometer to align optics, tune lasers, or evaluate fluidics performance—without needing biological material.
Available Variants
| Catalog Number | Laser Type | Bead Size | Excitation / Emission (nm) |
|---|---|---|---|
| A16500 | Blue Laser | 2.5 µm | 488 / 515–660 |
| A16501 | Red Laser | 2.5 µm | 630–640 / 645–680 |
| A16505 | UV Laser (also 405) | 6.0 µm | 350–370 / 400–470 |
Applications
- Instrument setup and daily QC
- Laser alignment and focusing
- Optical system performance verification
- Baseline calibration across cytometers
- Training and troubleshooting
Technical Specifications
| Property | Details |
|---|---|
| Product Name | AlignFlow™ Flow Cytometry Alignment Beads |
| Product Line | AlignFlow™, Molecular Probes™ |
| Format | Suspension (in dropper vials) |
| Bead Sizes Available | 2.5 µm or 6.0 µm (depending on variant) |
| Fluorescent Configuration | Internally stained (core label) |
| Laser Compatibility | UV, Violet, Blue, Red (depending on product) |
| Volume per Kit | 3 mL (2 × 1.5 mL vials) |
| Storage Conditions | 2–8°C; Do Not Freeze |
| Shipping Conditions | Room temperature |
| Instrument Compatibility | All flow cytometers |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Invitrogen™ AlignFlow™ Flow Cytometry Alignment Beads offer a reliable, reproducible, and convenient solution for ensuring optimal flow cytometer performance. Their laser-specific configurations, internal dye stability, and tight size distribution make them an essential QC tool for any cytometry laboratory seeking precision and consistency in instrument calibration.
- Laser: for Blue Lasers, 2.5 μm for Red Lasers, 2.5 μm for UV Lasers, 6.0 μm