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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Molecular Biology | ||
High-yield protein expression in insect cell cultures using the ExpiSf Expression System
The Gibco™ ExpiSf™ Protein Production Kit is an optimized solution for achieving maximum recombinant protein expression in high-density Sf9 insect cell cultures. Designed to support baculovirus-based expression systems, this kit includes ExpiSf CD Medium and the ExpiSf Enhancer—a unique additive that synergistically boosts protein yields when used in conjunction.
This animal origin-free, chemically defined medium system is scalable, from small-format deep-well plates to multi-liter shake flasks, ensuring flexibility across research, process development, and production environments.
Key Features
- Exceptional Yield: Achieves >80% increase in protein yield compared to ExpiSf CD Medium alone.
- Synergistic Enhancer: The ExpiSf Enhancer is specifically formulated to work with the CD Medium for maximum expression efficiency.
- Scalable System: Compatible with volumes ranging from 4 mL to >3 L, facilitating seamless process scale-up.
- Animal Origin-Free & Chemically Defined: Enhances reproducibility and reduces regulatory concerns.
- Designed for ExpiSf9 Cells: Optimized for use within the complete ExpiSf Expression System.
Applications
- High-yield protein production in Sf9 insect cells
- Research and development of baculovirus expression vectors
- Recombinant protein expression for structural biology, vaccine research, and biopharmaceutical development
Kit Components
| Catalog Number | ExpiSf CD Medium | ExpiSf Enhancer | Culture Volume Supported |
|---|---|---|---|
| A3767806 | 1 L | 4 × 1 mL | 1 L |
| A3767809 | 6 × 1 L | 24 × 1 mL | 6 L |
| A3767807 | 10 L | 40 mL | 10 L |
| A3767808 | 5 × 10 L | 5 × 40 mL | 50 L |
Technical Specifications
| Specification | Details |
|---|---|
| Classification | Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free |
| Compatible Cells | Insect cells (ExpiSf9) |
| Expression System | Baculovirus |
| Expression Mechanism | Cell-based expression |
| Format | Liquid |
| Shipping Conditions | Shipped at room temperature or on wet ice |
| Product Line | ExpiSf™ |
| Unit Size | Varies (see catalog numbers) |
Ordering Information
| Catalog Number | Configuration |
|---|---|
| A3767806 | 1 L Medium + 4 × 1 mL Enhancer |
| A3767809 | 6 × 1 L Medium + 24 × 1 mL Enhancer |
| A3767807 | 10 L Medium + 40 mL Enhancer |
| A3767808 | 5 × 10 L Medium + 5 × 40 mL Enhancer |
The Gibco™ ExpiSf™ Protein Production Kit provides a high-efficiency platform for maximizing protein yield from insect cells using the baculovirus system. Its chemically defined, scalable components make it an ideal solution for both research and production laboratories requiring robust and reproducible protein expression.
- Pack Size: 1 L (ExpiSf CD Medium), 4 x 1 mL (ExpiSf Enhancer) 6 x 1000 mL (ExpiSf CD Medium), 24 x 1 mL (ExpiSf Enhancer) 10 L (ExpiSf CD Medium), 40 mL (ExpiSf Enhancer) 5 x 10 L (ExpiSf CD Medium), 5 x 40 mL (ExpiSf Enhancer)