Thermo Scientific™ Eco130I (StyI) (10 U/μL), 2500 U

Catalog No :

CAS Number :

Brand :

Availability :

In Stock

Terms and Conditions
30-day money-back guarantee
Shipping: 2-3 Business Days

    This combination does not exist.

    Place Inquiry

    This combination does not exist.

    Specifications:
    Application Cloning
    Storage Temperature -20°C
    Product Type Enzymes Forms Liquid
    Product Brand Thermo Fisher Scientific™
    Product Grade Molecular Biology

    The Thermo Scientific™ Eco130I (StyI) is a high-quality restriction endonuclease that specifically recognizes the palindromic DNA sequence 5′-C^CWWGG-3′ (where W = A or T) and cleaves between the first two cytosines. This conventional restriction enzyme delivers high cleavage accuracy and reliability for a broad range of molecular biology applications, including cloning, RFLP analysis, and SNP detection.

    Eco130I (StyI) performs optimally at 37°C in 10X Buffer O, and is compatible with Thermo Scientific’s Five Buffer System, including the universal Tango buffer for efficient double digestions. The enzyme exhibits 100% activity in the recommended buffer, and is not sensitive to common forms of DNA methylation (Dam, Dcm, CpG).

    Also available in a FastDigest™ format for rapid protocols.

    Recognition & Cleavage Site

    mathematica

    CopyEdit

    5′ C ↓ C W W G G 3′ 3′ G G W W C ↑ C 5′

    (W = A or T; cleavage indicated by arrows)

    Key Features & Benefits

    • High Specificity – Recognizes and cleaves 5′-C^CWWGG-3′ with precision.
    • Reliable Performance – 100% activity in recommended 10X Buffer O.
    • Methylation-Insensitive – Not affected by Dam, Dcm, or CpG methylation.
    • Streamlined Workflow – Part of the Thermo Scientific Five Buffer System.
    • Double Digestion Flexibility – Includes universal Tango™ buffer for multi-enzyme reactions.
    • Premixed BSA in Buffers – Stabilizes enzymes and neutralizes contaminants.

    Applications

    • Molecular cloning
    • DNA mapping and restriction site analysis
    • Genotyping and SNP identification
    • Southern blot probe preparation
    • Restriction Fragment Length Polymorphism (RFLP) studies
    • Construction of recombinant DNA molecules

    Technical Specifications

    ParameterDetails
    Enzyme NameEco130I (StyI)
    Recognition Site5′-C^CWWGG-3′
    Cleavage PatternAfter first cytosine (C^CWWGG)
    IsoschizomersBssT1I, EcoT14I, ErhI, StyI
    Optimal Temperature37°C
    Reaction Buffer10X Buffer O
    Heat InactivationYes
    Methylation SensitivityNot Dam/Dcm/CpG sensitive
    Enzyme TypeTraditional Type II (not Type IIS)
    Concentration10 U/μL
    Total Units2,500 Units
    FormatConventional enzyme (not FastDigest)

    Storage & Handling

    • Storage Temperature: –20°C
    • Shipping: On dry ice
    • Stability: Long-term activity retained under recommended conditions


    The Thermo Scientific™ Eco130I (StyI) restriction enzyme is a dependable tool for molecular biologists conducting routine cloning or complex genotyping studies. Its robust performance, insensitivity to common methylation patterns, and compatibility with Thermo’s color-coded buffer system make it especially suited for workflows that demand flexibility and reproducibility. For researchers seeking precise cleavage at C^CWWGG sites, Eco130I offers a proven and high-quality solution.

    Product Specification not found.


    Add a Review

    Rate this Product  
    Name *
    Email *

    Your Review *
    30331





    0

    0  Reviews
    (0) 
    (0) 
    (0) 
    (0) 
    (0) 

    Customer Review




    Showing 0 Of 0