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Invitrogen™ Human VEGF Receptor 1 (Soluble) ELISA Kit, 96-Test Plate
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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Human VEGF Receptor 1 (Soluble) ELISA Kit (BMS268-3) is a highly sensitive and specific sandwich ELISA assay designed for quantitative detection of human soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR-1 / FLT1) in serum, plasma, and cell culture supernatants. VEGFR-1 is a key regulator of angiogenesis and vascular development, making it an important biomarker in cancer, cardiovascular disease, inflammation, and angiogenesis research.
This kit offers excellent analytical sensitivity of 0.03 ng/mL and a dynamic range of 0.16–10.0 ng/mL, allowing accurate quantification of soluble VEGFR-1 across physiological and pathological concentrations. The assay uses a pre-coated 96-well microplate and optimized reagents to ensure reliable, reproducible results with minimal hands-on time.
The Invitrogen VEGFR-1 ELISA kit is ideal for biomarker studies, angiogenesis research, and molecular biology applications requiring precise measurement of soluble VEGFR-1 protein levels.
Key Features and Advantages
Ultra-High Sensitivity and Precision
- Analytical sensitivity: 0.03 ng/mL
- Assay range: 0.16–10.0 ng/mL
-
Excellent reproducibility:
- Intra-assay CV: 5.5%
- Inter-assay CV: 5.1%
Highly Specific Sandwich ELISA Format
- Pre-coated capture antibody ensures target specificity
- Biotinylated detection antibody with HRP signal amplification
- Accurate quantification of soluble VEGFR-1
Optimized Sample Compatibility
- Requires only 50 µL sample volume
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Compatible sample types:
- Serum
- Plasma
- Cell culture supernatant
- Buffered biological solutions
Efficient and Reliable Workflow
- Hands-on time: 1 hour 20 minutes
- Total assay time: 3 hours 30 minutes
- Compatible with standard absorbance microplate readers
Validated and Quality-Assured Performance
- Each lot tested for sensitivity, specificity, and precision
- Ensures reliable and reproducible experimental results
Applications
The Human VEGFR-1 ELISA Kit is ideal for research in:
- Angiogenesis and vascular biology
- Cancer and tumor progression research
- Cardiovascular disease studies
- Inflammation and immune response research
- Biomarker discovery and validation
- Cell culture and protein expression studies
- Wound healing and tissue regeneration research
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Human VEGF Receptor 1 (Soluble) ELISA Kit |
| Brand | Invitrogen™ |
| Catalog Number | BMS268-3 |
| Assay Format | Sandwich ELISA |
| Analytical Sensitivity | 0.03 ng/mL |
| Assay Range | 0.16–10.0 ng/mL |
| Sample Types | Serum, Plasma, Cell Culture Supernatant |
| Sample Volume | 50 µL |
| Detection Method | HRP colorimetric detection |
| Detector Antibody | Biotin-conjugated |
| Plate Format | Pre-coated 96-well plate |
| Instrument Required | Absorbance microplate reader |
| Assay Time | 3 hr 30 min |
| Storage Conditions | 2–8°C |
| Shipping Conditions | Wet ice |
| Species Reactivity | Human |
| Research Use | Research Use Only (RUO) |
Kit Contents
- Pre-coated 96-well microplate
- VEGFR-1 standards
- Sample diluent
- Assay buffer concentrate
- Biotinylated detection antibody
- Streptavidin-HRP conjugate
- Wash buffer
- Chromogen substrate
- Stop solution
- Adhesive plate covers
Ordering Information
| Catalog Number | Product Name | Number of Tests | Pack Size |
|---|---|---|---|
| BMS268-3 | Human VEGF Receptor 1 (Soluble) ELISA Kit | 96 tests | 1 plate |
Target Information: VEGFR-1 (FLT1)
VEGF Receptor 1 (VEGFR-1), also known as FLT1, is a receptor tyrosine kinase involved in:
- Regulation of angiogenesis
- Endothelial cell growth and survival
- Tumor angiogenesis and metastasis
- Cardiovascular development and disease
Soluble VEGFR-1 acts as a decoy receptor that binds VEGF, regulating vascular growth and angiogenic signaling.
Unique Advantages
- Ultra-sensitive detection of soluble VEGFR-1
- Validated sandwich ELISA for accurate quantification
- High specificity for natural and recombinant VEGFR-1
- Reliable and reproducible performance
- Ready-to-use kit with optimized reagents
- Ideal for angiogenesis and biomarker research
The Invitrogen™ Human VEGF Receptor 1 (Soluble) ELISA Kit (BMS268-3) provides a reliable, sensitive, and efficient solution for quantifying soluble VEGFR-1 in human biological samples. With validated performance, excellent sensitivity, and optimized workflow, this kit is an essential tool for angiogenesis, cancer, cardiovascular, and molecular biology research.