Invitrogen™ Human MMP-9 ELISA Kit

The Invitrogen™ Human MMP-9 ELISA Kit is a highly sensitive and specific sandwich ELISA assay designed for quantitative measurement of human Matrix Metalloproteinase-9 (MMP-9) in serum, plasma, cell culture supernatants, and buffered solutions. This kit provides reliable and reproducible detection of both natural and recombinant human MMP-9, making it ideal for research in inflammation, cancer biology, cardiovascular disease, and tissue remodeling.

With an analytical sensitivity of 0.05 ng/mL and assay range of 0.23–15.0 ng/mL, the kit ensures accurate detection across physiological and pathological concentration ranges. The assay is optimized for efficiency, requiring only 10 µL sample volume and delivering results in approximately 3 hours and 10 minutes.

Each kit includes a pre-coated microplate, validated reagents, and optimized buffers, enabling researchers to perform consistent, high-quality quantitative ELISA assays using a standard absorbance microplate reader.

Key Features and Advantages

High Sensitivity and Precision

• Analytical sensitivity: 0.05 ng/mL
• Wide dynamic range: 0.23–15.0 ng/mL
• Excellent reproducibility:
  • Intra-assay CV: 7.3%
  • Inter-assay CV: 10.2%

Optimized Sandwich ELISA Format

• Pre-coated capture antibody ensures high specificity
• Biotin-labeled detection antibody with HRP-based signal amplification
• Accurate quantification of human MMP-9 protein levels

Minimal Sample Volume Requirement

• Requires only 10 µL sample volume
• Compatible with multiple sample types:
  • Serum
  • Plasma
  • Cell culture supernatants
  • Buffered solutions

Fast and Efficient Workflow

• Hands-on time: 1 hour 20 minutes
• Total assay time: 3 hours 10 minutes
• Suitable for high-throughput laboratory workflows

Fully Validated and Quality Tested

• Each lot validated for sensitivity, precision, and consistency
• Ensures reliable experimental results
• Manufactured under strict quality standards

Applications

This ELISA kit is ideal for research in:

• Cancer biology and tumor progression
• Inflammatory disease research
• Cardiovascular disease and vascular remodeling
• Tissue remodeling and wound healing studies
• Biomarker discovery and validation
• Immunology and cytokine research
• Cell culture and protein expression studies

Technical Specifications

Kit Contents

• Pre-coated 96-well microplate
• Standards
• Controls
• Sample diluent
• Assay buffer concentrate
• Biotinylated detection antibody
• Streptavidin-HRP conjugate
• Wash buffer
• Chromogen substrate
• Stop solution
• Adhesive plate covers

Target Information: MMP-9

Matrix Metalloproteinase-9 (MMP-9), also known as Gelatinase B, is a zinc-dependent endopeptidase involved in:

• Extracellular matrix degradation
• Tissue remodeling
• Tumor invasion and metastasis
• Inflammatory responses
• Angiogenesis and wound healing

Elevated MMP-9 levels are associated with:

• Cancer progression
• Cardiovascular diseases
• Inflammatory disorders
• Tissue injury and repair processes

Unique Advantages

• Ultra-sensitive detection of human MMP-9
• Validated sandwich ELISA format for accurate quantification
• Low sample volume requirement
• Reliable and reproducible performance
• Ready-to-use kit with optimized reagents
• Ideal for biomarker and disease research

The Invitrogen™ Human MMP-9 ELISA Kit (BMS2016-2) provides a highly sensitive, reliable, and efficient solution for quantifying human MMP-9 protein levels in biological samples. With validated performance, minimal sample requirements, and fast assay workflow, it is an essential tool for researchers studying cancer, inflammation, cardiovascular disease, and tissue remodeling.