Liofilchem ESBL+AMPC screening disc kit 50 tests

The Liofilchem™ ESBL+AmpC Screen Disc Kit is a diagnostic tool designed for the screening of ESBL (Extended-Spectrum Beta-Lactamase) and AmpC beta-lactamase-producing organisms. These resistance mechanisms are commonly found in Enterobacteriaceae, particularly Escherichia coli and Klebsiella pneumoniae. This kit utilizes Combination Disc Tests (CDT) to differentiate between ESBL, AmpC, and non-beta-lactamase-producing organisms.

Key Features

1. Dual Detection:
   • Differentiates ESBL and AmpC beta-lactamase production in a single assay.
2. Validated for Clinically Relevant Strains:
   • Includes Klebsiella pneumoniae, Escherichia coli, and Enterobacter cloacae.
3. CLSI and EUCAST Compliant:
   • Follows international guidelines for resistance mechanism detection.
4. Convenient Packaging:
   • Contains 4 x 50 discs, each packed in blisters with desiccants for optimal storage.

Kit Contents

• 4 x 50 disc cartridges:
  • CTX 30 μg: Cefotaxime.
  • CTL 30+10 μg: Cefotaxime + Clavulanic Acid.
  • CTC 30 μg: Cefotaxime + Cloxacillin.
  • CTLC 30+10 μg: Cefotaxime + Clavulanic Acid + Cloxacillin.

Method Principle

• Combination Disc Tests (CDT):
  • The test evaluates the inhibition zone sizes around Cefotaxime discs alone and in combination with inhibitors:
    • Clavulanic Acid: Inhibits ESBL beta-lactamases.
    • Cloxacillin: Inhibits AmpC beta-lactamases.
  • Inhibition zones are compared to detect and differentiate ESBL and AmpC production.

Test Procedure

1. Prepare Test Organism:
   • Use a pure, fresh culture to prepare a suspension equivalent to 0.5 McFarland Standard.
2. Plate Preparation:
   • Spread the bacterial suspension evenly over a Mueller Hinton II Agar plate using a sterile swab.
3. Disc Application:
   • Apply the discs (CTX, CTL, CTC, and CTLC) onto the plate, ensuring sufficient spacing for measurement.
4. Incubation:
   • Incubate the plate at 36 ± 1°C for 18–24 hours.
5. Measure and Interpret Results:
   • Measure inhibition zones and interpret based on the provided table.

Interpretation of Results

Quality Control

Storage and Shelf Life

• Storage:
  • Unopened blisters: -20°C to +8°C.
  • Opened cartridges: 2-8°C, for up to 7 days.
• Shelf Life: Valid until the expiry date indicated on the label.

Advantages

• Dual Screening: Simultaneously detects ESBL and AmpC production.
• Standardized: Compliant with CLSI and EUCAST guidelines for antimicrobial resistance detection.
• User-Friendly: Pre-packaged discs simplify laboratory workflows.
• Accurate Results: Reliable differentiation between resistance mechanisms.

Warnings and Precautions

1. For in vitro diagnostic use (IVD) only.
2. Handle all microbiological materials as potentially infectious.
3. Follow standard laboratory safety and aseptic techniques.
4. Dispose of expired discs and contaminated materials properly.

Packaging

References

1. Jacoby G.A. AmpC β-Lactamases. Clin Microbiol Rev. 2009; 22(1): 161–182.
2. EUCAST guidelines for resistance mechanism detection. Version 1.0, 2013.

Why Choose Liofilchem ESBL+AmpC Screen Disc Kit?

• Reliable Detection: Differentiates between ESBL and AmpC-producing organisms.
• Standardized Testing: Compliant with international guidelines for microbiological testing.
• Easy-to-Use Format: Pre-packaged discs simplify testing workflows in clinical laboratories.