0
Sigma-Aldrich Calbiochem® Y-27632, ≥95% (HPLC), liquid, Rho kinase inhibitor, 500 μg
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | Protein Biology, cell Signalling | ||
| Storage Temperature | -20°C | ||
| Product Type | Laboratory Chemical | Forms | Liquid |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Analytical grade | Formula | C₁₄H₂₁N₃O · 2HCl · xH₂O |
Y-27632 is a highly potent, cell-permeable, and selective Rho-associated coiled-coil-containing protein kinase (ROCK) inhibitor. It competitively binds to the ATP-binding site of ROCK-I (p160ROCK) and ROCK-II, key effectors in the RhoA/ROCK signaling pathway. Supplied as a convenient InSolution™ preparation, this reagent is suitable for direct use in cell culture and biochemical assays without reconstitution.
Key Specifications
| Property | Specification |
|---|---|
| Purity | ≥95% (HPLC) |
| Concentration | 5 mM (500 µg/296 µL H₂O) |
| Physical Form | Ready-to-use aqueous solution |
| Storage | −20°C (freeze after aliquoting) |
| Protect From Light | Yes |
| Cell Permeability | Yes |
| Reversible | No |
| Packaged | Under inert gas |
Biochemical Activity & Selectivity
- Primary Target: ROCK-I (p160ROCK) and ROCK-II
- Ki: 140 nM for ROCK-I
- IC₅₀: 700 nM for phenylephrine-induced Ca²⁺ sensitization in aortic smooth muscle
-
Selectivity Profile:
- 10–50× lower affinity for PKCε than for ROCK
- No activity against p21-activated kinase (PAK) up to 100 µM
Mechanism: ATP-competitive inhibition of ROCK catalytic domains, resulting in downstream inhibition of myosin light chain phosphorylation, stress fiber formation, and cellular contractility.
Applications in Research
- Cell migration and cytoskeletal remodeling
- Smooth muscle contraction inhibition
- Apoptosis and survival pathway studies
- Stem cell culture and reprogramming (e.g., maintenance of pluripotent stem cells)
- Wound healing and endothelial barrier function
- Cancer metastasis and invasion models
Advantages of InSolution™ Format
- Time-saving – no reconstitution needed
- Stability – aliquot and freeze for consistent dosing
- Reliability – consistent concentration, tested by HPLC, NMR, and MS
- Convenience – delivered in aqueous format, immediately compatible with culture systems
Storage & Handling
- Store at −20°C
- Protect from light to maintain compound stability
- After thawing, aliquot and refreeze to avoid multiple freeze-thaw cycles
- Product is stable for at least 6 months under recommended conditions
Literature References
- Uehata, M. et al. Nature 389, 990 (1997)
- Maekawa, M. et al. Science 285, 895 (1999)
- Davies, S.P. et al. Biochem J. 351, 95 (2000)
- Hirose, M. et al. J Cell Biol. 141, 1625 (1999)
Legal Information
Sold under license of PCT Application WO98/06433A1 and under license from Mitsubishi Pharma Tanabe Corporation.
Calbiochem® is a registered trademark of Merck KGaA, Darmstadt, Germany.
Calbiochem® Y-27632 (≥95%), in its ready-to-use InSolution™ liquid form, is a potent and highly selective ROCK inhibitor ideal for research on actin cytoskeleton dynamics, smooth muscle physiology, apoptosis, and stem cell maintenance. With validated purity, aqueous solubility, and superior ease-of-use, this reagent is a gold-standard compound for cell biology, pharmacology, and translational research labs.