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4-Nitrophenyl Phosphate Disodium Salt Hexahydrate (Pnpp) ≥98% Analytical Reagent, 5G
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Specifications:
| Application | molecular biology, Colorimetric Assay | ||
| Storage Temperature | -20°C | ||
| Product Type | Laboratory Chemical | Forms | Powder |
| Product Brand | VWR | ||
| Product Grade | Analytical grade | Formula | C₆H₄NNa₂O₆P·6H₂O |
The VWR® 4-Nitrophenyl Phosphate Disodium Salt Hexahydrate (pNPP) is a high-purity chromogenic substrate specifically designed for the quantitative determination of acid and alkaline phosphatase activity. Widely used in biochemical, clinical, and molecular biology laboratories, pNPP enables precise enzyme activity measurement through a simple and reliable colorimetric assay.
Upon enzymatic hydrolysis by phosphatases, pNPP is converted into p-nitrophenol, producing a yellow chromophore measurable at 405 nm, allowing accurate spectrophotometric quantification.
Key Features
- High purity (≥98%) for accurate and reproducible results
- Standard substrate for alkaline and acid phosphatase assays
- Chromogenic detection with strong absorbance at 405 nm
- Suitable for quantitative enzyme activity measurement
- Excellent solubility in water for easy preparation
- Stable when stored under recommended conditions
- Supplied in convenient solid format for flexible use
Applications
This reagent is ideal for:
- Alkaline phosphatase (ALP) activity assays
- Acid phosphatase enzyme assays
- Enzyme kinetics and inhibition studies
- Clinical and pharmaceutical research
- Molecular biology and cell-based assays
- Food safety and environmental enzyme analysis
Principle of Use
pNPP functions as a chromogenic substrate for phosphatases:
- Phosphatase enzymes hydrolyze pNPP
- p-nitrophenol (pNP) is released
- Under alkaline conditions, pNP produces a yellow color
- Absorbance is measured at 405 nm
- Color intensity is proportional to enzyme activity
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | 4-Nitrophenyl Phosphate Disodium Salt Hexahydrate (pNPP) |
| CAS Number | 333338-18-4 |
| Molecular Formula | C₆H₄NNa₂O₆P·6H₂O |
| Molecular Weight | 371.14 g/mol |
| Purity | ≥98% |
| Appearance | White to off-white crystalline powder |
| Solubility | Soluble in water |
| Detection Wavelength | 405 nm |
| Melting Point | >300°C |
| Storage | -20°C (recommended) |
Advantages
- Reliable substrate for phosphatase activity measurement
- High sensitivity and reproducibility
- Compatible with standard spectrophotometric assays
- Ideal for both research and routine laboratory testing
- High chemical stability when properly stored
Available Format
| Pack Type | Quantity | Catalog Number |
|---|---|---|
| Plastic bottle (solid) | 5 g | 27963.101 |
Handling & Storage
- Store at -20°C to maintain stability
- Protect from moisture and light
- Avoid repeated exposure to ambient conditions
- Follow appropriate laboratory safety procedures
The VWR® pNPP (4-Nitrophenyl Phosphate Disodium Salt Hexahydrate) is a trusted and widely used reagent for phosphatase enzyme assays, offering high purity, strong chromogenic response, and excellent reproducibility. It is an essential tool for biochemical analysis, enzymology studies, and clinical research applications.