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Specifications:
| Application | Gene Expression, Multiplex qPCR | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | Nucleic acid controls | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
TaqMan™ Custom DNA Controls are linearized multi-target plasmid pools designed for use as positive controls in microbial detection research workflows. They are suitable for use with TaqMan™ OpenArray™ plates and TaqMan™ Array Cards, supporting panel-specific amplification verification, assay performance monitoring, troubleshooting, and analytical validation.
These custom DNA controls can be included as positive controls to confirm that assays within a microbial detection panel are amplifying correctly. High-concentration formats are also useful for determining key analytical validation parameters such as limit of detection, Ct cut-off values, and assay performance consistency.
Key Features
| Feature | Description |
|---|---|
| Custom multi-target control | Linearized plasmid pool designed around panel-specific microbial targets |
| Positive control use | Helps confirm amplification performance for microbial detection panels |
| Compatible with TaqMan™ platforms | Designed for use with TaqMan™ OpenArray™ plates and TaqMan™ Array Cards |
| Supports assay validation | High-copy formats can be used for LoD and Ct cut-off studies |
| Troubleshooting support | Can be run as a stand-alone sample during real-time PCR to verify assay performance |
| Multiple target-range options | Available for panels with ≤20, 21–30, 31–50, and >50 targets |
| Two concentration formats | Available at 1 × 10⁵ copies/µL and 5 × 10⁷ copies/µL |
Product Variants
| Catalog Number | Description | Number of Targets | Concentration | Volume |
|---|---|---|---|---|
| A50319 | TaqMan™ Custom DNA Control | ≤20 targets | 1 × 10⁵ copies/µL | 1000 µL |
| A50375 | TaqMan™ Custom DNA Control | 21 to 30 targets | 1 × 10⁵ copies/µL | 1000 µL |
| A50376 | TaqMan™ Custom DNA Control | 31 to 50 targets | 1 × 10⁵ copies/µL | 1000 µL |
| A50377 | TaqMan™ Custom DNA Control | >50 targets | 1 × 10⁵ copies/µL | 1000 µL |
| A50378 | TaqMan™ Custom DNA Control | ≤20 targets | 5 × 10⁷ copies/µL | 50 µL |
| A50379 | TaqMan™ Custom DNA Control | 21 to 30 targets | 5 × 10⁷ copies/µL | 50 µL |
| A50380 | TaqMan™ Custom DNA Control | 31 to 50 targets | 5 × 10⁷ copies/µL | 50 µL |
| A50381 | TaqMan™ Custom DNA Control | >50 targets | 5 × 10⁷ copies/µL | 50 µL |
Applications
| Application Area | Use |
|---|---|
| Microbial detection research | Positive control for panel-specific amplification |
| TaqMan™ OpenArray™ workflows | Control material for microbial detection panels |
| TaqMan™ Array Card workflows | Control material for card-based real-time PCR panels |
| Assay performance verification | Confirms assay amplification and helps identify workflow issues |
| Analytical validation | Supports determination of LoD, Ct cut-off values, and assay performance parameters |
| Real-time PCR troubleshooting | Can be run as a stand-alone sample to verify assay performance |
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | TaqMan™ Custom DNA Controls |
| Product Type | Custom DNA Control |
| Control Format | Linearized multi-target plasmid pool |
| Workflow | Real-time PCR / microbial detection research |
| Compatible Platforms | TaqMan™ OpenArray™ plates, TaqMan™ Array Cards |
| Target Options | ≤20, 21–30, 31–50, and >50 targets |
| Concentration Options | 1 × 10⁵ copies/µL; 5 × 10⁷ copies/µL |
| Volume Options | 1000 µL; 50 µL |
| Storage | Store at –15°C to –25°C |
TaqMan™ Custom DNA Controls provide reliable, panel-specific positive control material for microbial detection research using TaqMan™ OpenArray™ plates and TaqMan™ Array Cards. Available across multiple target ranges and two concentration formats, they support assay verification, real-time PCR troubleshooting, and analytical validation studies, including limit of detection and Ct cut-off determination.
- Concentration & Volume: ≤20 targets, 1 × 10⁵ copies/µL, 1000 µL 21–30 targets, 1 × 10⁵ copies/µL, 1000 µL 31–50 targets, 1 × 10⁵ copies/µL, 1000 µL 50 targets, 1 × 10⁵ copies/µL, 1000 µL ≤20 targets, 5 × 10⁷ copies/µL, 50 µL 21–30 targets, 5 × 10⁷ copies/µL, 50 µL 31–50 targets, 5 × 10⁷ copies/µL, 50 µL 50 targets, 5 × 10⁷ copies/µL, 50 µL