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Specifications:
| Application | Cloning | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Efficient 5′-Phosphorylation of DNA and Oligonucleotides for Cloning Workflows
The Invitrogen™ Anza™ T4 PNK Kit is a fast and convenient enzyme system designed for the 5′-phosphorylation of DNA fragments and oligonucleotides, enabling efficient ligation into cloning vectors. T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the terminal phosphate group from ATP to the 5′-hydroxyl terminus of nucleic acids. This enzyme also exhibits 3′-phosphatase activity, enabling streamlined processing of DNA prior to blunt-end or sticky-end ligation.
The pre-formulated Anza™ 10X T4 PNK Buffer provides optimal conditions for activity and is compatible with the full Anza™ restriction enzyme cloning system.
Key Features and Benefits
- Fast Protocol – 5′-end phosphorylation completed in just 15 minutes at room temperature
- Versatile Substrate Compatibility – Works with DNA or oligonucleotides in water, TE, or 1X Anza buffers
- Streamlined Workflow – Ideal for pre-ligation preparation of PCR products or restriction fragments
- Part of the Anza™ Cloning System – Integrates with other DNA modifying tools like ligase and end repair kits
- Reliable and Consistent – Reduces reaction steps while maintaining cloning efficiency
Specifications
| Property | Value |
|---|---|
| Product Name | Anza™ T4 Polynucleotide Kinase (PNK) Kit |
| Catalog Number | IVGN2304 |
| Reaction Capacity | 50 reactions |
| Enzyme | T4 Polynucleotide Kinase (PNK) |
| Buffer | Anza™ 10X T4 PNK Buffer (included) |
| Compatible Buffers | Water, TE, 1X Anza Buffer |
| Shipping Conditions | Wet or Dry Ice |
| Storage Temperature | –5°C to –30°C |
| Product Format | Ready-to-use enzyme and buffer mix |
| Regulatory Status | For Research Use Only (RUO) |
Kit Contents
| Component | Volume |
|---|---|
| Anza T4 PNK Enzyme | 50 reactions |
| Anza 10X T4 PNK Buffer | 0.5 mL |
Applications
- Phosphorylation of 5′ ends on DNA or oligonucleotides
- Preparation of PCR products or synthesized DNA for ligation
- Pre-treatment of restriction fragments prior to cloning
- Molecular biology workflows requiring terminal modification
- Compatible with Anza™ Ligase and End Repair Kits for streamlined cloning
The Invitrogen™ Anza™ T4 PNK Kit provides a fast, high-quality solution for 5′-phosphorylation of DNA and oligonucleotides, facilitating efficient ligation in cloning protocols. Its ease of use, room temperature incubation, and compatibility with other Anza™ products make it an essential tool for modern molecular biology labs.