Invitrogen™ Anza™ T4 DNA Ligase Master Mix

The Invitrogen™ Anza™ T4 DNA Ligase Master Mix is a high-performance, ready-to-use enzyme mix that enables efficient ligation of 5’-phosphate and 3’-hydroxyl termini in duplex DNA. It is suitable for both sticky-end and blunt-end ligation, as well as nick repair in double-stranded DNA. As part of the Anza™ Restriction Enzyme Cloning System, this 4X concentrated master mix streamlines traditional cloning workflows by reducing pipetting steps and allowing ligation directly from DNA in TE, water, or elution buffer.

Key Features and Benefits

• Fast Ligation – Complete in as little as 15 minutes at room temperature
• Versatile – Supports sticky-end, blunt-end, and nick repair ligation
• Convenient Master Mix Format – Minimizes preparation steps and variability
• 4X Concentration – Enables use with dilute DNA without the need for prior concentration
• Compatible Buffers – DNA in water, TE, elution buffer, or 1X Anza™ buffers

Specifications

Applications

• Molecular Cloning – Vector-insert ligation (sticky or blunt ends)
• Nick Repair – Restoration of 3’-OH/5’-phosphate nicks in double-stranded DNA
• Gibson/Golden Gate Assembly Prep – General DNA fragment joining prior to cloning
• Synthetic Biology – Constructing plasmids and DNA libraries
• Restriction-Ligation Workflows – Optimized for Anza™ Restriction Enzyme Cloning System

Ordering Information

Invitrogen™ Anza™ T4 DNA Ligase Master Mix provides a streamlined, high-efficiency ligation solution compatible with a wide range of molecular biology applications. With its rapid performance and compatibility with common buffers and cloning workflows, it is an essential tool for researchers working with DNA ligation and cloning.