0
Thermo Scientific™ SuperBlock™ Blocking Buffer
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | ELISA-Based Assays, Immunohistochemistry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ SuperBlock™ Blocking Buffer is an optimized protein-based blocking solution designed for fast and effective blocking in ELISA, western blotting, immunohistochemistry (IHC), and membrane-based detection workflows. It contains a single purified glycoprotein that helps reduce nonspecific binding and improve signal-to-noise ratio across a wide range of assay systems.
SuperBlock™ Blocking Buffer is especially useful for blocking polystyrene microplates, membranes, and tissue samples. Its formulation is free from immunoglobulins, albumin, and endogenous biotin, making it suitable for applications involving streptavidin-biotin detection systems where traditional blockers may interfere.
Key Features
| Feature | Details |
|---|---|
| Fast Blocking Performance | Blocks membranes in 5–10 minutes and ELISA plates in approximately 2 minutes |
| High Signal-to-Noise Ratio | Helps reduce nonspecific background for clearer assay results |
| Broad Application Use | Suitable for ELISA, western blotting, and IHC workflows |
| Biotin-Free Formulation | Compatible with streptavidin-biotin detection systems |
| Protein-Based Blocking | Contains a purified glycoprotein without immunoglobulins or albumin |
| Plate Stabilization | Effective for blocking coated polystyrene microplates and supporting dry storage |
| Ready-to-Use Options | Available in PBS, TBS, and Tween-20-containing formulations |
Applications
Thermo Scientific™ SuperBlock™ Blocking Buffer is suitable for:
- ELISA plate blocking
- Western blot membrane blocking
- Immunohistochemistry blocking workflows
- Blocking coated polystyrene microplates
- Reducing nonspecific antibody binding
- Streptavidin-biotin assay systems
- Immunoassay development and optimization
- Stabilization of blocked plates for drying and storage
- Research workflows requiring high signal-to-noise performance
Product Specifications
| Specification | Details |
|---|---|
| Product Type | Blocking buffer |
| Product Line | SuperBlock™ |
| Concentration | 1X |
| Physical Form | Liquid |
| Primary Application | ELISA |
| Recommended Storage | Store at 4 °C upon receipt |
| Shipping Condition | Ambient |
| Unit Size | Each |
| Research Use | For Research Use Only; not for diagnostic procedures |
Ordering Information
| Catalog Number | Quantity | Chemical Name or Material |
|---|---|---|
| 37516 | 1 L | SuperBlock™ T20 Blocking Buffer in PBS |
| 37580 | 100 mL | SuperBlock™ Blocking Buffer in PBS |
| 37515 | 1 L | SuperBlock™ Blocking Buffer in PBS |
| 37518 | 5 L | SuperBlock™ Blocking Buffer in PBS |
| 37581 | 100 mL | SuperBlock™ Blocking Buffer in TBS |
| 37535 | 1 L | SuperBlock™ Blocking Buffer in TBS |
| 37545 | 5 Pk. | SuperBlock™ Blocking Buffer in TBS, Dry Blend |
| 37536 | 1 L | SuperBlock™ T20 Blocking Buffer in TBS |
Storage & Handling
Store at 4 °C upon receipt. Product is shipped at ambient temperature. Keep container tightly closed when not in use and avoid contamination during repeated use. SuperBlock™ Buffer can be stored at 4 °C for up to one year, and blocked plates may be dried and stored for up to 12 months under appropriate conditions.
Research Use Statement
For Research Use Only (RUO). Not for use in diagnostic procedures.
- Buffer Type: SuperBlock™ T20 Blocking Buffer in PBS, 1 L SuperBlock™ Blocking Buffer in PBS, 100 mL SuperBlock™ Blocking Buffer in PBS, 1 L SuperBlock™ Blocking Buffer in PBS, 5 L SuperBlock™ Blocking Buffer in TBS, 100 mL SuperBlock™ Blocking Buffer in TBS, 1 L SuperBlock™ Blocking Buffer in TBS, Dry Blend, 5 Pk. SuperBlock™ T20 Blocking Buffer in TBS, 1 L