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Thermo Scientific™ Pierce™ High-Capacity Ni-IMAC MagBeads, EDTA Compatible
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Specifications:
| Application | Magnetic Affinity Separation, Protein Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | ImmunoMagnetic Separation Products | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Pierce™ High Capacity Ni-IMAC MagBeads, EDTA Compatible are magnetic affinity beads designed for efficient purification of overexpressed His-tagged proteins, especially from cell culture media and expression systems where EDTA or reducing agents may be present.
These beads offer high protein binding capacity, strong chelator stability, and convenient magnetic handling for both manual and automated purification workflows. They are particularly useful for purification of secreted His-tagged proteins from systems such as Expi293™, ExpiCHO™, and ExpiSf™ expression platforms.
Ordering Information
| Catalog Number | Quantity |
|---|---|
| A50588 | 1 mL |
| A50589 | 5 mL |
| A50590 | 25 mL |
| A50591 | 100 mL |
Key Features
| Feature | Benefit |
|---|---|
| EDTA compatible | Stable in buffers containing up to 20 mM EDTA |
| Reducing agent compatible | Stable in buffer conditions containing DTT |
| High binding capacity | Binds up to 80 mg of 6xHis-tagged protein per mL of settled beads |
| Magnetic agarose format | Enables rapid purification without columns or centrifugation |
| Suitable for secreted proteins | Compatible with overexpressed His-tagged proteins in cell culture media |
| Manual or automated use | Works with magnetic stands and KingFisher™ magnetic particle processors |
| Non-aggregating superparamagnetic beads | Supports consistent performance in high-throughput workflows |
| Broad pH stability | Stable across pH 2 to 14 |
Applications
These Ni-IMAC MagBeads are suitable for:
- His-tagged recombinant protein purification
- Purification of secreted proteins from cell culture media
- Small-scale protein purification
- High-throughput protein purification
- Expression screening
- Purification condition scouting
- Manual magnetic purification workflows
- Automated purification using KingFisher™ systems
- Workflows requiring EDTA or reducing agents
- Expi293™, ExpiCHO™, and ExpiSf™ protein expression workflows
Technology Overview
Pierce™ High Capacity Ni-IMAC MagBeads consist of crosslinked agarose beads embedded with magnetite. The magnetic bead format enables fast separation using a magnetic stand or automated magnetic processor.
The beads are designed with a chelator-stable Ni-IMAC chemistry that maintains performance in buffer systems containing EDTA and reducing agents. This makes them especially valuable for sensitive intracellular proteins or protein workflows where metalloprotease inhibition and protein stabilization are required.
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Thermo Scientific™ Pierce™ High Capacity Ni-IMAC MagBeads, EDTA Compatible |
| Product Line | Pierce™ |
| Type | Magnetic Affinity Bead |
| Matrix | Magnetic agarose |
| Form | Liquid suspension |
| Concentration | 25% slurry |
| Particle Size | 30 µm |
| Binding Capacity | Up to 80 mg 6xHis-tagged protein/mL settled beads |
| Binding Property | Chelator stable |
| Column Type | Bottle |
| pH Stability | pH 2 to 14 |
| Shipping Condition | Wet ice |
| Storage | 2–8°C |
| Unit Size | Each |
Thermo Scientific™ Pierce™ High Capacity Ni-IMAC MagBeads, EDTA Compatible provide a high-capacity magnetic purification solution for His-tagged recombinant proteins. Their EDTA compatibility, reducing agent tolerance, high binding capacity, and compatibility with manual or automated workflows make them ideal for laboratories purifying sensitive proteins from cell culture media or screening purification conditions at small scale and high throughput.
- Pack Size: 25 mL 100 mL 1 mL 5 mL