Apollo Sci. Imidazole

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Specifications:
Application cell Signalling
Storage Temperature Ambient
Product Type Antifungals Forms Powder
Product Brand Apollo Scientific
Product Grade Molecular Biology Formula C₃H₄N₂

Imidazole is a heteroaromatic organic compound with a five-membered ring structure containing two nitrogen atoms. It is a white to off-white crystalline solid with a wide range of applications in biochemistry, molecular biology, pharmaceuticals, and catalysis. Imidazole is widely used in protein purification workflows, particularly in Ni-NTA affinity chromatography for the elution of His-tagged proteins.

Key Features

  • High purity Imidazole (≥99%).
  • Available in 500 g, 1 kg, and 2.5 kg bottles.
  • Essential for Ni-NTA protein purification (His-tag elution).
  • Used in the synthesis of pharmaceuticals, agrochemicals, and fine chemicals.
  • Functions as a buffering agent in biochemical applications.

Specifications

ParameterDetails
Product NameImidazole
CAS Number288-32-4
Molecular FormulaC₃H₄N₂
Molecular Weight68.08 g/mol
MDL NumberMFCD00005183
EINECS Number206-019-2
Purity≥99%
AppearanceWhite to off-white crystalline solid
Melting Point85–91 °C
Boiling Point235 °C
Flash Point145 °C
Storage TemperatureAmbient
UN Number3263
ADR ClassificationClass 8 (Corrosive), Packing Group II
Hazard StatementsCorrosive (H314), Irritant, Health hazard
Packaging Options500 g (OR5102-500G), 1 kg (OR5102-1KG), 2.5 kg (R5102-2.5KG)

Applications

  • Protein purification: Imidazole competes with histidine residues to release His-tagged proteins in affinity chromatography.
  • Biochemical research: Used as a buffering agent in enzymatic assays.
  • Pharmaceutical synthesis: A building block for antifungals, antivirals, and other therapeutic agents.
  • Catalysis and materials science: Ligand for transition metal complexes and ionic liquids.
  • Agriculture: Precursor in the production of agrochemicals.

Safety Information

  • Classified as Corrosive and Irritant.
  • May cause skin burns, eye damage, and respiratory irritation.
  • Handle with gloves, goggles, and protective clothing.
  • Store in tightly sealed containers at ambient temperature.
  • UN 3263, ADR Class 8, Packing Group II.


Imidazole (CAS 288-32-4) is a versatile heteroaromatic compound used in biochemistry, pharmaceuticals, catalysis, and industrial chemistry. Its critical role in His-tagged protein purification makes it indispensable in molecular biology and biotechnology research. With high purity, multiple pack sizes, and broad applications, this reagent is a reliable choice for academic, industrial, and pharmaceutical laboratories.

  • Pack Size:   1kg 500g 2.5kg
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