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Thermo Scientific™ Pierce™ 96-Well Maleic Anhydride Activated Plates
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Specifications:
| Application | Colorimetric Assay, Peptide Synthesis |
| Material | Polystyrene |
| Storage Temperature | Room Temperature |
| Product Type | Plate |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
Thermo Scientific™ Pierce™ Maleic Anhydride Activated Plates are clear 96-well polystyrene microplates designed for the covalent immobilization of amine-containing peptides, proteins, ligands, and other biomolecules. These plates are especially useful for molecules that do not bind efficiently through passive adsorption.
The maleic anhydride surface reacts spontaneously with primary amines (-NH₂) to form covalent amide bonds. This provides a direct and simple method for attaching target molecules to the well surface for ELISA, EIA, binding assays, screening workflows, and other colorimetric assay applications.
Key Features
| Feature | Details |
|---|---|
| Maleic Anhydride Activated Surface | Reacts with primary amines for covalent immobilization |
| Covalent Binding | Enables stable attachment of peptides and ligands at neutral pH and above |
| Clear 96-Well Format | Suitable for colorimetric detection and absorbance-based assays |
| Ideal for Poor Passive Binders | Useful for peptides and molecules that do not coat efficiently by adsorption |
| Simple Coating Workflow | Provides a direct approach to surface coupling |
| Room Temperature Stability | Maleic anhydride surface remains available for coupling for months under dry room-temperature storage |
| Flexible Formats | Available as standard plates and strip plates |
Applications
Pierce™ Maleic Anhydride Activated Plates are suitable for:
- ELISA and EIA workflows
- Colorimetric binding assays
- Covalent immobilization of peptides
- Attachment of amine-containing ligands
- Peptide-protein and protein-ligand interaction studies
- Screening assay development
- Assays requiring stronger attachment than passive adsorption
- Research workflows involving primary amine coupling
Product Specifications
| Specification | Details |
|---|---|
| Product Type | Microplate |
| Product Line | Pierce™ |
| Binding Property | Standard capacity, covalent |
| Surface Treatment | Maleic Anhydride |
| Detection Method | Colorimetric |
| Color | Clear |
| No. of Wells | 96 |
| Material | Polystyrene |
| Plate Blocking | None |
| No. per Pack | 5 plates |
| Unit Size | Each |
| Research Use | For Research Use Only; not for diagnostic procedures |
Ordering Information
| Catalog Number | Color | Format | Quantity |
|---|---|---|---|
| 15100 | Clear | Strip Plate | 5 Plates |
| 15102 | Clear | Strip Plate | 5 x 5 Plates |
| 15110 | Clear | Standard | 5 Plates |
| 15112 | Clear | Standard | 5 x 5 Plates |
Usage Notes
Coating reactions are best performed at pH 8–9, where maleic anhydride groups efficiently react with primary amines. The resulting amide bonds are stable at neutral pH and above. Acidic conditions can hydrolyze the bond, so reacted plates are best used at pH greater than 7 for ELISA and related assay workflows.
Storage & Handling
Upon receipt, store plates at room temperature. Keep plates dry and protected from moisture until use. Product is shipped at ambient temperature.
Research Use Statement
For Research Use Only (RUO). Not for use in diagnostic procedures.
- Plate Type: Strip Plate, Clear, 5 Plates Strip Plate, Clear, 5 x 5 Plates Standard Plate, Clear, 5 Plates Standard Plate, Clear, 5 x 5 Plates