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Thermo Scientific™ Pierce™ HeLa Protein Digest Standard
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Specifications:
| Application | Protein Assays & Analysis, Mass Spectrometry | ||
| Storage Temperature | -20°C | ||
| Product Type | Calibration Standard | Forms | Lyophilized |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Analytical grade | ||
Thermo Scientific™ Pierce™ HeLa Protein Digest Standard is a highly validated mammalian protein digest standard designed for quality control and performance monitoring in complex proteomic mass spectrometry workflows. Derived from the well-established HeLa S3 cell line, this lyophilized tryptic peptide mixture provides a complex proteome reference sample containing more than 15,000 proteins.
The standard is prepared using LysC and trypsin digestion to improve digestion efficiency, reduce missed cleavages, and support reliable LC/MS method development, system suitability testing, and long-term performance benchmarking.
Key Features
| Feature | Description |
|---|---|
| Complex mammalian proteome standard | Derived from HeLa S3 cells expressing more than 15,000 proteins |
| Positive control sample | Suitable for routine LC/MS quality control and performance monitoring |
| Thorough enzymatic digestion | Prepared using both LysC and trypsin for improved sequence coverage |
| Low missed cleavage rate | Designed to ensure less than 10% tryptic missed cleavages |
| Validated peptide quality | Less than 10% methionine oxidation and less than 10% lysine carbamylation |
| Lot-specific documentation | Supplied with lot-specific certificates of analysis |
| Lyophilized format | Stable solid format for convenient storage and handling |
| MS-compatible formulation | Does not contain salts or detergents that may interfere with LC/MS analysis |
Product Variants
| Catalog Number | Product Description | Quantity |
|---|---|---|
| 88329 | Pierce™ HeLa Protein Digest Standard | 5 × 20 µg |
| 88328 | Pierce™ HeLa Protein Digest Standard | 20 µg |
Applications
| Application Area | Use |
|---|---|
| LC/MS quality control | Routine monitoring of LC/MS system performance |
| LC assessment | Qualitative assessment of liquid chromatography separation |
| MS method development | Development and optimization of mass spectrometry methods |
| LC/MS standardization | Standardization of LC/MS performance across runs and instruments |
| Performance benchmarking | Monitoring and normalizing instrument performance over time |
| TMT™ reagent method development | Method development for TMT-based quantitative proteomics workflows |
| Proteomics workflow validation | Control sample for complex proteomic sample analysis |
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Thermo Scientific™ Pierce™ HeLa Protein Digest Standard |
| Catalog Numbers | 88329, 88328 |
| Product Type | Protein Digest Standard |
| Product Line | Pierce™ |
| Reagent Type | Standard |
| Final Product Type | Peptides |
| Starting Material | Peptides |
| Form | Solid, lyophilized |
| Detection Method | Mass Spectrometry |
| For Use With | Mass Spectrometer |
| Workflow Step | MS system performance |
| Quantity Options | 20 µg; 5 × 20 µg |
| Unit Size | Each |
| Use Statement | For Research Use Only. Not for use in diagnostic procedures |
Unique Advantages
| Advantage | Benefit |
|---|---|
| Highly complex reference digest | Provides a realistic mammalian proteome standard for challenging LC/MS workflows |
| Consistent lot-to-lot performance | Supports reproducible benchmarking and long-term system monitoring |
| Validated digestion quality | Helps ensure reliable peptide representation and reduced digestion variability |
| Free from salts and detergents | Suitable for direct use in LC/MS experiments after preparation |
| Supports routine system suitability testing | Enables laboratories to monitor performance before analyzing valuable samples |
Thermo Scientific™ Pierce™ HeLa Protein Digest Standard is a reliable and highly validated LC/MS quality control standard for proteomics laboratories. With its complex HeLa S3-derived peptide mixture, low missed cleavage rate, validated peptide quality, and stable lyophilized format, it is ideal for LC/MS method development, system standardization, performance benchmarking, and routine quality control in mass spectrometry-based proteomics research.
- Pack Size: 1 x 20 μg 5 x 20 μg